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Literature summary for 2.7.1.107 extracted from

  • Tu-Sekine, B.; Ostroski, M.; Raben, D.M.
    Modulation of diacylglycerol kinase theta activity by alpha-thrombin and phospholipids (2007), Biochemistry, 46, 924-932.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidic acid more effective activator than phosphatidylserine. Phosphatidic acid decreases the apparent surface KM of DGKtheta for dioleoylglycerol and promotes binding to vesicles in a dose-dependent manner Homo sapiens
phosphatidic acid phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms Homo sapiens
phosphatidylserine less effective activator than phosphatidic acid. Phosphatidylserine decreases the apparent surface KM of DGKtheta for dioleoylglycerol Homo sapiens
phosphatidylserine phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in IIC9 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha-thrombin apparent binding parameters of diacylglycerol kinase theta increase following alpha-thrombin stimulation Homo sapiens
thrombin stimulation results in an increase in the apparent KM of DGKtheta at low concentrations of substrate dioleoylglycerol. Increasing the bulk concentration of dioleoylglycerol returns the apparent KM to the basal value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KMapp for substrate 1,2-dioleoyl-sn-glycerol is 0.9 mol% in presence of 1% phosphatidic acid, 0.6 mol% in presence of 9% phosphatidylserine, and 8.0 mol% without additive Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-dioleoyl-sn-glycerol
-
Homo sapiens ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
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