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Literature summary for 2.7.1.107 extracted from

  • Dicu, A.O.; Matthew, K.; Topham, K.; Ottaway, L.; Epand, R.M.
    Role of the hydrophobic segment of diacylglycerol kinase epsilon (2007), Biochemistry, 46, 6109-6117.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells Homo sapiens
expression of truncated enzyme forms in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information truncated form of the protein (DGKDELTAepsilon) lacking the 40 N-terminal amino acids. Full-length FLAG-DGKepsilon and truncated FLAG-DGKepsilon are both more specific for 1-stearoyl-2-arachidonoyl-sn-glycerol than for 1,2-dioleoyl-sn-glycerol. 1-Stearoyl-2-linoleoyl-sn-glycerol exhibits intermediate specificity for both forms of the enzyme. The truncated form of the enzyme maintains substrate specificity for lipids with an arachidonoyl moiety present at the sn-2 position. The truncation increases the catalytic rate constant for all three substrates and may suggest a role in the negative regulation of this enzyme Homo sapiens
additional information expression of truncated FLAG-tagged protein lacking the 40 N-terminal amino acids, which includes the hydrophobic segment. Truncated protein maintains substrate specificity and increases catalytic rate constant. Truncated protein may be extracted with 1.5 M KCl at neutral pH value, while wild-type protein remains fully membrane bound Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information turnover numbers of full-length DGKepsilon with a C-terminal His tag, full-length FLAG-DGKepsilon and truncated FLAG-DGKepsilon Homo sapiens
0.13
-
ATP wild-type protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
0.135
-
ATP N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
0.2
-
ATP wild-type protein with C-terminal His-tag, pH 7.2, 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the hydrophobic domain of DGKepsilo does not contribute to substrate specificity but plays a role in permanently sequestering the enzyme to a membrane Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
truncated protein lacking the 40 N-terminal amino acids may be extracted with 1.5 M KCl at neutral pH value, while wild-type protein remains fully membrane bound Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-dioleoyl-sn-glycerol
-
Homo sapiens ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
Homo sapiens ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-linoleoyl-sn-glycerol
-
Homo sapiens ADP + 1-stearoyl-2-linoleoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer GKepsilon is monomeric on SDS-PAGE but exhibits partial dimerization with low concentrations of perfluorooctanoic acid Homo sapiens
More wild-type protein has a weak tendency to oligomerize in presence of weak detergents Homo sapiens

Synonyms

Synonyms Comment Organism
DGKepsilon
-
Homo sapiens
diacylglycerol kinase
-
Homo sapiens
epsilonDGK
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information turnover numbers of full-length DGKepsilon with a C-terminal His tag, full-length FLAG-DGKepsilon and truncated FLAG-DGKepsilon Homo sapiens
0.01
-
ATP wild-type protein with C-terminal His-tag, pH 7.2, 25°C Homo sapiens
0.032
-
1,2-dioleoyl-sn-glycerol wild-type protein with C-terminal His-tag, pH 7.2, 25°C Homo sapiens
0.087
-
1-stearoyl-2-linoleoyl-sn-glycerol wild-type protein with C-terminal His-tag, pH 7.2, 25°C Homo sapiens
0.162
-
1-stearoyl-2-arachidonoyl-sn-glycerol wild-type protein with C-terminal His-tag, pH 7.2, 25°C Homo sapiens
1.2
-
1,2-dioleoyl-sn-glycerol wild-type protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
1.23
-
1-stearoyl-2-linoleoyl-sn-glycerol wild-type protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
1.7
-
1-stearoyl-2-arachidonoyl-sn-glycerol wild-type protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
2.43
-
1,2-dioleoyl-sn-glycerol N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
2.8
-
1-stearoyl-2-linoleoyl-sn-glycerol N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
5.7
-
1-stearoyl-2-arachidonoyl-sn-glycerol N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
8.2
-
ATP wild-type protein, FLAG-tag, pH 7.2, 25°C Homo sapiens
23
-
ATP N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C Homo sapiens