Cloned (Comment) | Organism |
---|---|
expression in COS-7 cells | Homo sapiens |
expression of truncated enzyme forms in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated form of the protein (DGKDELTAepsilon) lacking the 40 N-terminal amino acids. Full-length FLAG-DGKepsilon and truncated FLAG-DGKepsilon are both more specific for 1-stearoyl-2-arachidonoyl-sn-glycerol than for 1,2-dioleoyl-sn-glycerol. 1-Stearoyl-2-linoleoyl-sn-glycerol exhibits intermediate specificity for both forms of the enzyme. The truncated form of the enzyme maintains substrate specificity for lipids with an arachidonoyl moiety present at the sn-2 position. The truncation increases the catalytic rate constant for all three substrates and may suggest a role in the negative regulation of this enzyme | Homo sapiens |
additional information | expression of truncated FLAG-tagged protein lacking the 40 N-terminal amino acids, which includes the hydrophobic segment. Truncated protein maintains substrate specificity and increases catalytic rate constant. Truncated protein may be extracted with 1.5 M KCl at neutral pH value, while wild-type protein remains fully membrane bound | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers of full-length DGKepsilon with a C-terminal His tag, full-length FLAG-DGKepsilon and truncated FLAG-DGKepsilon | Homo sapiens | |
0.13 | - |
ATP | wild-type protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
0.135 | - |
ATP | N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
0.2 | - |
ATP | wild-type protein with C-terminal His-tag, pH 7.2, 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the hydrophobic domain of DGKepsilo does not contribute to substrate specificity but plays a role in permanently sequestering the enzyme to a membrane | Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
truncated protein lacking the 40 N-terminal amino acids may be extracted with 1.5 M KCl at neutral pH value, while wild-type protein remains fully membrane bound | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-dioleoyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | - |
Homo sapiens | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-linoleoyl-sn-glycerol | - |
Homo sapiens | ADP + 1-stearoyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | GKepsilon is monomeric on SDS-PAGE but exhibits partial dimerization with low concentrations of perfluorooctanoic acid | Homo sapiens |
More | wild-type protein has a weak tendency to oligomerize in presence of weak detergents | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DGKepsilon | - |
Homo sapiens |
diacylglycerol kinase | - |
Homo sapiens |
epsilonDGK | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers of full-length DGKepsilon with a C-terminal His tag, full-length FLAG-DGKepsilon and truncated FLAG-DGKepsilon | Homo sapiens | |
0.01 | - |
ATP | wild-type protein with C-terminal His-tag, pH 7.2, 25°C | Homo sapiens | |
0.032 | - |
1,2-dioleoyl-sn-glycerol | wild-type protein with C-terminal His-tag, pH 7.2, 25°C | Homo sapiens | |
0.087 | - |
1-stearoyl-2-linoleoyl-sn-glycerol | wild-type protein with C-terminal His-tag, pH 7.2, 25°C | Homo sapiens | |
0.162 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | wild-type protein with C-terminal His-tag, pH 7.2, 25°C | Homo sapiens | |
1.2 | - |
1,2-dioleoyl-sn-glycerol | wild-type protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
1.23 | - |
1-stearoyl-2-linoleoyl-sn-glycerol | wild-type protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
1.7 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | wild-type protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
2.43 | - |
1,2-dioleoyl-sn-glycerol | N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
2.8 | - |
1-stearoyl-2-linoleoyl-sn-glycerol | N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
5.7 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
8.2 | - |
ATP | wild-type protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens | |
23 | - |
ATP | N-terminally trucated protein, FLAG-tag, pH 7.2, 25°C | Homo sapiens |