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Literature summary for 2.7.1.107 extracted from

  • Jose, A.M.; Koelle, M.R.
    Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo (2005), J. Biol. Chem., 280, 2730-2736.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dgk-1alpha, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, expression of N-terminally His-tagged wild-type and mutant enzymes and of wild-type enzyme enzyme fused to the maltose binding protein in insect cells via the baculovirus infection system, enzyme is found to 95% in aggregated form Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
A722V mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
C115Y mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
C184Y mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
G606E mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
G609E mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
G796R mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
additional information determination of mutational defects/molecular lesions affecting the enzyme activity and splice forms of the enzyme, overview Caenorhabditis elegans
N745I mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
P736S mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
Q246stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
Q422stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
R167stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
R180stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
S880L mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
W646stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
W674stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans
W767stop mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Caenorhabditis elegans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ at 10 mM MgCl2 Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacylglycerol Caenorhabditis elegans
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
additional information Caenorhabditis elegans the enzyme inhibits neurotransmission to control behaviour by terminating diacylglycerol signaling, probably independent of Galpha0 signaling ?
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
different splice forms
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type and mutant enzymes or MBP-fusion protein from insect cells by nickel affinity chromatography Caenorhabditis elegans

Renatured (Commentary)

Renatured (Comment) Organism
solubilization of the active enzyme from aggregates after recombinant expression in yeast Caenorhabditis elegans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00001
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1-oleoyl-rac-glycerol Caenorhabditis elegans
0.0008
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1,3-dioleoyl-sn-glycerol Caenorhabditis elegans
0.0019
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1,2-dioleoyl-sn-glycerol Caenorhabditis elegans
0.0021
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1-stearoyl-2-arachidonoyl-sn-glycerol Caenorhabditis elegans
7.5
-
purified solubilized MBP-fusion wild-type enzyme Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacylglycerol
-
Caenorhabditis elegans ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-dioleoyl-sn-glycerol
-
Caenorhabditis elegans ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
?
ATP + 1,3-dioleoyl-sn-glycerol low activity, about 4% of the activity with 1,2-dioleoyl-sn-glycerol Caenorhabditis elegans ADP + 1,3-dioleoyl-sn-glycerol 2-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol about 110% of the activity with 1,2-dioleoyl-sn-glycerol Caenorhabditis elegans ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
additional information the enzyme inhibits neurotransmission to control behaviour by terminating diacylglycerol signaling, probably independent of Galpha0 signaling Caenorhabditis elegans ?
-
?
additional information 1-oleoyl-rac-glycerol is a poor substrate Caenorhabditis elegans ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
DGK-1
-
Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
ATP
-
Caenorhabditis elegans