Cloned (Comment) | Organism |
---|---|
gene dgk-1alpha, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, expression of N-terminally His-tagged wild-type and mutant enzymes and of wild-type enzyme enzyme fused to the maltose binding protein in insect cells via the baculovirus infection system, enzyme is found to 95% in aggregated form | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
A722V | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
C115Y | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
C184Y | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
G606E | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
G609E | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
G796R | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
additional information | determination of mutational defects/molecular lesions affecting the enzyme activity and splice forms of the enzyme, overview | Caenorhabditis elegans |
N745I | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
P736S | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
Q246stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
Q422stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
R167stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
R180stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
S880L | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
W646stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
W674stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
W767stop | mutant isolated due to defects in DGK-1 controlled behaviour, altered behaviour compared to the wild-type enzyme, overview | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Caenorhabditis elegans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | at 10 mM MgCl2 | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | Caenorhabditis elegans | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
additional information | Caenorhabditis elegans | the enzyme inhibits neurotransmission to control behaviour by terminating diacylglycerol signaling, probably independent of Galpha0 signaling | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
different splice forms | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type and mutant enzymes or MBP-fusion protein from insect cells by nickel affinity chromatography | Caenorhabditis elegans |
Renatured (Comment) | Organism |
---|---|
solubilization of the active enzyme from aggregates after recombinant expression in yeast | Caenorhabditis elegans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00001 | - |
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1-oleoyl-rac-glycerol | Caenorhabditis elegans |
0.0008 | - |
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1,3-dioleoyl-sn-glycerol | Caenorhabditis elegans |
0.0019 | - |
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1,2-dioleoyl-sn-glycerol | Caenorhabditis elegans |
0.0021 | - |
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1-stearoyl-2-arachidonoyl-sn-glycerol | Caenorhabditis elegans |
7.5 | - |
purified solubilized MBP-fusion wild-type enzyme | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | - |
Caenorhabditis elegans | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-dioleoyl-sn-glycerol | - |
Caenorhabditis elegans | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,3-dioleoyl-sn-glycerol | low activity, about 4% of the activity with 1,2-dioleoyl-sn-glycerol | Caenorhabditis elegans | ADP + 1,3-dioleoyl-sn-glycerol 2-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | about 110% of the activity with 1,2-dioleoyl-sn-glycerol | Caenorhabditis elegans | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | the enzyme inhibits neurotransmission to control behaviour by terminating diacylglycerol signaling, probably independent of Galpha0 signaling | Caenorhabditis elegans | ? | - |
? | |
additional information | 1-oleoyl-rac-glycerol is a poor substrate | Caenorhabditis elegans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
DGK-1 | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Caenorhabditis elegans |