Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged or FLAG-tagged wild-type and mutant isozymes theta in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G236R | site-directed mutagenesis, highly reduced activity compared to the wild-type isozyme theta | Homo sapiens |
L241V | site-directed mutagenesis, slightly reduced activity compared to the wild-type isozyme theta | Homo sapiens |
additional information | construction of deletion mutants, N- or C-terminal truncations inactivate the isozyme theta | Homo sapiens |
P244A | site-directed mutagenesis, reduced activity compared to the wild-type isozyme theta | Homo sapiens |
P244L | site-directed mutagenesis, reduced activity compared to the wild-type isozyme theta | Homo sapiens |
P245L | site-directed mutagenesis, highly reduced activity compared to the wild-type isozyme theta | Homo sapiens |
S240T | site-directed mutagenesis, activity is unaltered compared to the wild-type isozyme theta | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RhoA | V14-RhoA, strong binding to the C-terminal catalytic domain, negative regulation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | Homo sapiens | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme theta | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate | conserved residues in the extended cysteine-rich domain CRD are essential for activity. e.g. G236, P244, and P245 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | - |
Homo sapiens | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
r | |
ATP + 1,2-diacylglycerol | 1,2-diacylglycerol is a second messenger | Homo sapiens | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? |
Subunits | Comment | Organism |
---|---|---|
More | isozyme theta is the only one of 9 isozyme forms, that possesses three instead of two cysteine-rich domains at the N-terminal regulatory region | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DGK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |