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Literature summary for 2.7.1.107 extracted from

  • Los, A.P.; van Baal, J.; de Widt, J.; Divecha, N.; van Blitterswijk, W.J.
    Structure-activity relationship of diacylglycerol kinase theta (2004), Biochim. Biophys. Acta, 1636, 169-174.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged or FLAG-tagged wild-type and mutant isozymes theta in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
G236R site-directed mutagenesis, highly reduced activity compared to the wild-type isozyme theta Homo sapiens
L241V site-directed mutagenesis, slightly reduced activity compared to the wild-type isozyme theta Homo sapiens
additional information construction of deletion mutants, N- or C-terminal truncations inactivate the isozyme theta Homo sapiens
P244A site-directed mutagenesis, reduced activity compared to the wild-type isozyme theta Homo sapiens
P244L site-directed mutagenesis, reduced activity compared to the wild-type isozyme theta Homo sapiens
P245L site-directed mutagenesis, highly reduced activity compared to the wild-type isozyme theta Homo sapiens
S240T site-directed mutagenesis, activity is unaltered compared to the wild-type isozyme theta Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
RhoA V14-RhoA, strong binding to the C-terminal catalytic domain, negative regulation Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacylglycerol Homo sapiens
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme theta
-

Reaction

Reaction Comment Organism Reaction ID
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate conserved residues in the extended cysteine-rich domain CRD are essential for activity. e.g. G236, P244, and P245 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacylglycerol
-
Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
r
ATP + 1,2-diacylglycerol 1,2-diacylglycerol is a second messenger Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Subunits

Subunits Comment Organism
More isozyme theta is the only one of 9 isozyme forms, that possesses three instead of two cysteine-rich domains at the N-terminal regulatory region Homo sapiens

Synonyms

Synonyms Comment Organism
DGK
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens