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Literature summary for 2.7.1.107 extracted from

  • Clark, E.H.; East, J.M.; Lee, A.G.
    The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase (2003), Biochemistry, 42, 11065-11073.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes Escherichia coli

Protein Variants

Protein Variants Comment Organism
W112L site-directed mutagenesis, inactive mutant Escherichia coli
W117L site-directed mutagenesis, inactive mutant Escherichia coli
W18L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
W18L/W25L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
W18L/W25L/W112L/W117L site-directed mutagenesis, inactive mutant Escherichia coli
W18L/W25L/W47L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
W18L/W25L/W47L/W112L site-directed mutagenesis, inactive mutant Escherichia coli
W18L/W25L/W47L/W117L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
W18L/W47L/W112L/W117L site-directed mutagenesis, inactive mutant Escherichia coli
W18L/W47L/W117L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
W25L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
W25L/W47L/W112L/W117L site-directed mutagenesis, inactive mutant Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, recombinant wild-type and mutant enzymes Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacylglycerol Escherichia coli
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HIs-tagged wild-type and mutant enzymes Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23
-
purified recombinant mutant W25L Escherichia coli
26
-
purified recombinant mutant W18L/W25L/W47L Escherichia coli
36
-
purified recombinant mutant W18L/W25L/W47L/W117L Escherichia coli
42
-
purified recombinant mutant W18L/W25L Escherichia coli
61
-
purified recombinant mutant W18L Escherichia coli
77
-
purified recombinant mutant W18L/W47L/W117L Escherichia coli
102
-
purified recombinant wild-type enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacylglycerol
-
Escherichia coli ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
r
ATP + 1,2-diacylglycerol
-
Escherichia coli ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid r

Synonyms

Synonyms Comment Organism
DGK
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ADP
-
Escherichia coli
ATP
-
Escherichia coli