Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
W112L | site-directed mutagenesis, inactive mutant | Escherichia coli |
W117L | site-directed mutagenesis, inactive mutant | Escherichia coli |
W18L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Escherichia coli |
W18L/W25L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Escherichia coli |
W18L/W25L/W112L/W117L | site-directed mutagenesis, inactive mutant | Escherichia coli |
W18L/W25L/W47L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Escherichia coli |
W18L/W25L/W47L/W112L | site-directed mutagenesis, inactive mutant | Escherichia coli |
W18L/W25L/W47L/W117L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Escherichia coli |
W18L/W47L/W112L/W117L | site-directed mutagenesis, inactive mutant | Escherichia coli |
W18L/W47L/W117L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Escherichia coli |
W25L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Escherichia coli |
W25L/W47L/W112L/W117L | site-directed mutagenesis, inactive mutant | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant wild-type and mutant enzymes | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | Escherichia coli | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant HIs-tagged wild-type and mutant enzymes | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23 | - |
purified recombinant mutant W25L | Escherichia coli |
26 | - |
purified recombinant mutant W18L/W25L/W47L | Escherichia coli |
36 | - |
purified recombinant mutant W18L/W25L/W47L/W117L | Escherichia coli |
42 | - |
purified recombinant mutant W18L/W25L | Escherichia coli |
61 | - |
purified recombinant mutant W18L | Escherichia coli |
77 | - |
purified recombinant mutant W18L/W47L/W117L | Escherichia coli |
102 | - |
purified recombinant wild-type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | - |
Escherichia coli | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
r | |
ATP + 1,2-diacylglycerol | - |
Escherichia coli | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | r |
Synonyms | Comment | Organism |
---|---|---|
DGK | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Escherichia coli | |
ATP | - |
Escherichia coli |