Protein Variants | Comment | Organism |
---|---|---|
A14Q | significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes, subunit mixing experiments of mutant enzymes | Escherichia coli |
D95N | significantly impaired catalytic function, without evidence of gross structural alterations. Km-value for MgATP2- raises 18fold, subunit mixing experiments of mutant enzymes | Escherichia coli |
E69C | mutant enzyme has an altered structure even in SDS | Escherichia coli |
E76L | significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes | Escherichia coli |
K94L | significantly impaired catalytic function, without evidence of gross structural alterations. Km-value for MgATP2- raises 13fold, subunit mixing experiments of mutant enzymes | Escherichia coli |
N72S | significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.12 | - |
ATP | wild-type enzyme | Escherichia coli | |
0.12 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
0.14 | - |
ATP | mutant enzyme E76L | Escherichia coli | |
0.14 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
0.33 | - |
ATP | mutant enzyme E69C | Escherichia coli | |
0.33 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
0.44 | - |
ATP | mutant enzyme N72S | Escherichia coli | |
0.44 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
0.91 | - |
ATP | mutant enzyme A14Q | Escherichia coli | |
0.91 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
1.5 | - |
ATP | mutant enzyme K94V | Escherichia coli | |
1.5 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
2.1 | - |
ATP | mutant enzyme D95N | Escherichia coli | |
2.1 | - |
ATP | ATP in form of MgATP2- | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | - |
Escherichia coli | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |