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Literature summary for 2.7.1.107 extracted from

  • Kanoh, H.; Kondoh, H.; Ono, T.
    Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies (1983), J. Biol. Chem., 258, 1767-1774.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate enhances activity Sus scrofa
lysophosphatidylcholine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
phosphatidylcholine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
phosphatidylethanolamine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
phosphatidylserine activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
Phospholipid enhances activity. Activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa
sphingomyelin activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
phosphatidate moderate Sus scrofa
phosphatidylglycerol inhibits phosphatidylcholine-dependent kinase activity Sus scrofa
phosphatidylinositol 2.5 mol% results in 50% inhibition of the phosphatidylcholine-dependent kinase activity Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ATP pH 7.4, 30°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
gel filtration Sus scrofa
78000
-
1 * 78000, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week Sus scrofa
4°C, purified enzyme, 50% loss of activity after 3 days Sus scrofa

Subunits

Subunits Comment Organism
monomer 1 * 78000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
phosphatidylcholine-dependent activity Sus scrofa
8
-
broad, deoxycholate-dependent or phosphatidylcholine-dependent activity Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.5: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, phosphatidylcholine-dependent activity Sus scrofa