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Literature summary for 2.7.1.107 extracted from

  • Yada, Y.; Ozeki, T.; Kanoh, H.; Nozawa, Y.
    Purification and characterization of cytosolic diacylglycerol kinases of human platelets (1990), J. Biol. Chem., 265, 19237-19243.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate enhances activity of enzyme form DGK-II and DGK-III, enzyme form DGK-I is not much affected Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
octyl glucoside enzyme form DGK-I, DGK-II and DGK-III Homo sapiens
phosphatidylcholine enzyme form DGK-I, DGK-II and DGK-III Homo sapiens
R59022 inhibits DGK-II and to a lesser extent DGK-III, but little affects DGK-I Homo sapiens
Triton N-101 enzyme form DGK-I, DGK-II and DGK-III Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
diolein pH 7.4, 30°C, enzyme form DGK-I Homo sapiens
0.065
-
diolein pH 7.4, 30°C, enzyme form DGK-II Homo sapiens
0.08
-
diolein pH 7.4, 30°C, enzyme form DGK-III Homo sapiens
0.15
-
ATP pH 7.4, 30°C, enzyme form DGK-I Homo sapiens
0.245
-
ATP pH 7.4, 30°C, enzyme form DGK-II Homo sapiens
0.45
-
ATP pH 7.4, 30°C, enzyme form DGK-III Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoenzyme DGK-I, DGK-II and DGK-II Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal activity at 2.5 mM for DGK-I, 5.0 mM for DGK-II and 10.0 mM for DGK-III Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, enzyme form DGK-III, SDS-PAGE Homo sapiens
75000
-
enzyme form DGK-II, gel filtration Homo sapiens
152000
-
x * 152000, enzyme form DGK-I, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme DGK-I and DGK-II, DGK-II only partially purified Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
platelet isoenzyme DGK-I, DGK-II and DGK-II Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.207
-
enzyme form DGK-II Homo sapiens
4.74
-
enzyme form DGK-I Homo sapiens
6.753
-
enzyme form DGK-III Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2,3-dioleoyl-sn-glycerol
-
Homo sapiens ADP + 2,3-diacyl-sn-glycerol 1-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 152000, enzyme form DGK-I, SDS-PAGE Homo sapiens
? x * 58000, enzyme form DGK-III, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
5 min, complete loss of activity of DGK-II and DGK-III, about 10% loss of activity of enzyme form DGK-I Homo sapiens