Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | enhances activity of enzyme form DGK-II and DGK-III, enzyme form DGK-I is not much affected | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
octyl glucoside | enzyme form DGK-I, DGK-II and DGK-III | Homo sapiens | |
phosphatidylcholine | enzyme form DGK-I, DGK-II and DGK-III | Homo sapiens | |
R59022 | inhibits DGK-II and to a lesser extent DGK-III, but little affects DGK-I | Homo sapiens | |
Triton N-101 | enzyme form DGK-I, DGK-II and DGK-III | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
diolein | pH 7.4, 30°C, enzyme form DGK-I | Homo sapiens | |
0.065 | - |
diolein | pH 7.4, 30°C, enzyme form DGK-II | Homo sapiens | |
0.08 | - |
diolein | pH 7.4, 30°C, enzyme form DGK-III | Homo sapiens | |
0.15 | - |
ATP | pH 7.4, 30°C, enzyme form DGK-I | Homo sapiens | |
0.245 | - |
ATP | pH 7.4, 30°C, enzyme form DGK-II | Homo sapiens | |
0.45 | - |
ATP | pH 7.4, 30°C, enzyme form DGK-III | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoenzyme DGK-I, DGK-II and DGK-II | Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal activity at 2.5 mM for DGK-I, 5.0 mM for DGK-II and 10.0 mM for DGK-III | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, enzyme form DGK-III, SDS-PAGE | Homo sapiens |
75000 | - |
enzyme form DGK-II, gel filtration | Homo sapiens |
152000 | - |
x * 152000, enzyme form DGK-I, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme DGK-I and DGK-II, DGK-II only partially purified | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
platelet | isoenzyme DGK-I, DGK-II and DGK-II | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.207 | - |
enzyme form DGK-II | Homo sapiens |
4.74 | - |
enzyme form DGK-I | Homo sapiens |
6.753 | - |
enzyme form DGK-III | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2,3-dioleoyl-sn-glycerol | - |
Homo sapiens | ADP + 2,3-diacyl-sn-glycerol 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 152000, enzyme form DGK-I, SDS-PAGE | Homo sapiens |
? | x * 58000, enzyme form DGK-III, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
5 min, complete loss of activity of DGK-II and DGK-III, about 10% loss of activity of enzyme form DGK-I | Homo sapiens |