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Literature summary for 2.7.1.107 extracted from

  • Schaap, D.; de Widt, J.; van der Wal, J.; Vandekerckhove, J.; van Damme, J.; Gussow, D.; Ploegh, H.L.; van Blitterswijk, W.J.; van der Bend, R.L.
    Purification, cDNA-cloning and expression of human diacylglycerol kinase (1990), FEBS Lett., 275, 151-158.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine. Diacylglycerol analogs containing an unsaturated fatty acid at the sn-2 position give optimal enzyme activity irrespective of the presence of deoxycholate Homo sapiens
phosphatidylcholine
-
Escherichia coli
phosphatidylcholine the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine Homo sapiens
phosphatidylserine
-
Escherichia coli
phosphatidylserine the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
subcloning into the expression vector pMT-2 and transfection in COS-7 cells results in a 6-7fold increase in diacylglycerol kinase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
1,2-diolein pH 7.5, 30°C Homo sapiens
0.5
-
ATP pH 7.5, 30°C, without deoxycholate Homo sapiens
1.6
-
ATP pH 7.5, 30°C, with 1 mM deoxycholate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-
membrane bound to Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
Homo sapiens
-
T-lymphocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.913
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-dipalmitoyl-sn-glycerol 15% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-O-hexadecyl-2-oleoyl-sn-glycerol 45.4% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 1-O-hexadecyl-2-oleoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-palmitoyl-2-oleoyl-sn-glycerol 96.5% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
Homo sapiens ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide Homo sapiens ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
ATP + 2,3-dioleoyl-sn-glycerol
-
Homo sapiens ADP + 2,3-diacyl-sn-glycerol 1-phosphate
-
?
ATP + 2-monooleoyl-rac-glycerol 10.7% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 2-monooleoyl-rac-glycerol 3-phosphate
-
?
ATP + sn-1,2-dioleoylglycerol
-
Escherichia coli ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
?
ATP + sn-1,2-dioleoylglycerol
-
Homo sapiens ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer
-
Escherichia coli
monomer 1 * 86000, SDS-PAGE Homo sapiens