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Literature summary for 2.7.1.105 extracted from

  • Cullen, K.S.; Al-Oanzi, Z.H.; OHarte, F.P.; Agius, L.; Arden, C.
    Glucagon induces translocation of glucokinase from the cytoplasm to the nucleus of hepatocytes by transfer between 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2 and the glucokinase regulatory protein (2014), Biochim. Biophys. Acta, 1843, 1123-1134.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.105

Protein Variants

Protein Variants Comment Organism
additional information generation of a fluorescent PFK2/FBPase2-YC155 construct Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
dibutyryl cAMP slightly inhibits the complex formation between the enzyme and glucokinase Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the glucokinase-PFK2/FBPase2 complex predominately localizes to the cytoplasmic compartment, peripheral location of the glucokinase-PFK2/FBPase2 interaction close to the plasma membrane Rattus norvegicus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Rattus norvegicus
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
ATP + beta-D-fructose 6-phosphate Rattus norvegicus Wistar
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
additional information Rattus norvegicus the enzyme binds to glucokinase in the cytoplasm ?
-
 ?
additional information Rattus norvegicus Wistar the enzyme binds to glucokinase in the cytoplasm ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P07953 gene Pfkfb1
-
Rattus norvegicus Wistar P07953 gene Pfkfb1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of PFK2/FBPase2 on Ser32 by protein kinase A Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
 Rattus norvegicus
-
liver liver isozyme Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus Wistar ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
additional information the enzyme binds to glucokinase in the cytoplasm Rattus norvegicus ?
-
 ?
additional information the enzyme binds to glucokinase in the cytoplasm Rattus norvegicus Wistar ?
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2
-
 Rattus norvegicus
PFK2/FBPase2
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

General Information

General Information Comment Organism
metabolism glucose causes decreased binding of glucokinase to glucokinase regulatory protein, GKRP, translocation from the nucleus and increased binding to 6-phosphofructo 2-kinase/fructose 2,6 bisphosphatase-2 (PFK2/FBPase2) in the cytoplasm, while glucagon causes dissociation of glucokinase from PFK2/FBPase2, concomitant with phosphorylation of PFK2/FBPase2 on Ser32, uptake of glucokinase into the nucleus and increased interaction with GKRP Rattus norvegicus
physiological function role for phosphofructo 2-kinase/fructose 2,6-bisphosphate (PFK2/FBPase2) as a cytoplasmic binding partner of glucokinase and glucagon-induced uptake of glucokinase to the nucleus Rattus norvegicus