Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a fluorescent PFK2/FBPase2-YC155 construct | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dibutyryl cAMP | slightly inhibits the complex formation between the enzyme and glucokinase | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the glucokinase-PFK2/FBPase2 complex predominately localizes to the cytoplasmic compartment, peripheral location of the glucokinase-PFK2/FBPase2 interaction close to the plasma membrane | Rattus norvegicus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Rattus norvegicus | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | Rattus norvegicus Wistar | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
additional information | Rattus norvegicus | the enzyme binds to glucokinase in the cytoplasm | ? | - |
? | |
additional information | Rattus norvegicus Wistar | the enzyme binds to glucokinase in the cytoplasm | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P07953 | gene Pfkfb1 | - |
Rattus norvegicus Wistar | P07953 | gene Pfkfb1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of PFK2/FBPase2 on Ser32 by protein kinase A | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Rattus norvegicus | - |
liver | liver isozyme | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus Wistar | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
additional information | the enzyme binds to glucokinase in the cytoplasm | Rattus norvegicus | ? | - |
? | |
additional information | the enzyme binds to glucokinase in the cytoplasm | Rattus norvegicus Wistar | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2 | - |
Rattus norvegicus |
PFK2/FBPase2 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | glucose causes decreased binding of glucokinase to glucokinase regulatory protein, GKRP, translocation from the nucleus and increased binding to 6-phosphofructo 2-kinase/fructose 2,6 bisphosphatase-2 (PFK2/FBPase2) in the cytoplasm, while glucagon causes dissociation of glucokinase from PFK2/FBPase2, concomitant with phosphorylation of PFK2/FBPase2 on Ser32, uptake of glucokinase into the nucleus and increased interaction with GKRP | Rattus norvegicus |
physiological function | role for phosphofructo 2-kinase/fructose 2,6-bisphosphate (PFK2/FBPase2) as a cytoplasmic binding partner of glucokinase and glucagon-induced uptake of glucokinase to the nucleus | Rattus norvegicus |