Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in enzyme-deficient strain DF1020 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C238A | site-directed mutagenesis, the mutation does not affect the kinetic parameters, allosteric inhibition, dimer stability, nor oligomeric structure of the enzyme | Escherichia coli |
C238F | site-directed mutagenesis, the mutation does not affect the kinetic parameters, allosteric inhibition, dimer stability, nor oligomeric structure of the enzyme | Escherichia coli |
C295A | site-directed mutagenesis, decreased kcat and increased Km for ATP and beta-D-fructose 6-phosphate compared to the wild-type enzyme | Escherichia coli |
C295F | site-directed mutagenesis, decreased kcat and increased Km for ATP and beta-D-fructose 6-phosphate compared to the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgATP2- | allosteric inhibition, important regulation of in vivo carbohydrate metabolism under gluconeogenic conditions | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes | Escherichia coli | |
0.0107 | - |
MgATP2- | recombinant mutant C295F | Escherichia coli | |
0.017 | - |
MgATP2- | recombinant wild-type enzyme and mutant C238A | Escherichia coli | |
0.021 | - |
MgATP2- | recombinant mutant C238F | Escherichia coli | |
0.031 | - |
beta-D-fructose 6-phosphate | recombinant wild-type enzyme and mutant C238A | Escherichia coli | |
0.047 | - |
beta-D-fructose 6-phosphate | recombinant mutant C238F | Escherichia coli | |
0.061 | - |
MgATP2- | recombinant mutant C295A | Escherichia coli | |
0.081 | - |
beta-D-fructose 6-phosphate | recombinant mutant C295F | Escherichia coli | |
0.088 | - |
beta-D-fructose 6-phosphate | recombinant mutant C295A | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | as MgATP2- | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Escherichia coli | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
additional information | Escherichia coli | Cys-295 is involved in allosteric regulation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from enzyme-deficient strain DF1020 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Escherichia coli | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
MgATP2- + beta-D-fructose 6-phosphate | - |
Escherichia coli | ? | - |
? | |
additional information | Cys-295 is involved in allosteric regulation | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | active form | Escherichia coli |
More | oligomeric state of the enzyme plays a role in enzyme activity and regulation, alterations occur via binding of MgATP2- which mediates dimer-dimer interactions, the dimeric state of the enzyme is critical for stability and activity of the enzyme, Cys-295 plays a role in subunit interactions, overview | Escherichia coli |
tetramer | inactive form | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Pfk-2 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
beta-D-fructose 6-phosphate | recombinant mutant C295A | Escherichia coli | |
13 | - |
MgATP2- | recombinant mutant C295F | Escherichia coli | |
39 | - |
MgATP2- | recombinant mutant C295A | Escherichia coli | |
39 | - |
beta-D-fructose 6-phosphate | recombinant mutant C295F | Escherichia coli | |
70 | - |
MgATP2- | recombinant mutants C238A and C238F | Escherichia coli | |
70 | - |
beta-D-fructose 6-phosphate | recombinant mutants C238A and C238F | Escherichia coli | |
90 | - |
MgATP2- | recombinant wild-type enzyme | Escherichia coli | |
90 | - |
beta-D-fructose 6-phosphate | recombinant wild-type enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | as MgATP2- | Escherichia coli |