Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme | Rattus norvegicus |
additional information | mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoenolpyruvate | - |
Bos taurus | |
phosphoenolpyruvate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
beta-D-fructose 6-phosphate | pH 7.5, 25°C, heart | Bos taurus | |
0.023 | - |
beta-D-fructose 6-phosphate | comparison of Km of wild-type, mutant and mutant phosphorylated enzyme | Rattus norvegicus | |
0.023 | - |
beta-D-fructose 6-phosphate | comparison of Km of wild-type, mutant and mutant phosphorylated enzyme | Bos taurus | |
0.025 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C, liver, comparison of Km of wild-type, mutant and mutant phosphorylated enzyme | Rattus norvegicus | |
0.04 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C, wild-type enzyme, testis | Rattus norvegicus | |
0.061 | - |
ATP | pH 7.5, 30°C, liver | Rattus norvegicus | |
0.083 | - |
ATP | pH 7.5, 25°C, heart | Bos taurus | |
0.1 | - |
ATP | - |
Bos taurus | |
0.1 | - |
ATP | pH 7.5, 30°C, testis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme | Rattus norvegicus |
mutant enzyme | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Rattus norvegicus | |
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
heart | - |
Bos taurus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
ATP + beta-D-fructose 6-phosphate | - |
Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Rattus norvegicus | ? | - |
? | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
25 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of kcat/Km values of wild-type and mutant enzymes | Rattus norvegicus | |
additional information | - |
additional information | comparison of kcat/Km values of wild-type and mutant enzymes | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of Ki of wild type and mutant enzymes | Rattus norvegicus | |
0.014 | - |
phosphoenolpyruvate | pH 7.5, 30°C, testis | Rattus norvegicus | |
0.08 | - |
phosphoenolpyruvate | pH 7.5, 30°C, liver | Rattus norvegicus | |
0.1 | - |
phosphoenolpyruvate | pH 7.5, 30°C, heart | Bos taurus |