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Literature summary for 2.7.1.105 extracted from

  • Harmsen, H.J.M.; Kubicek-Pranz, E.M.; Röhr, M.; Visser, J.; Kubicek, C.P.
    Regulation of 6-phosphofructo-2-kinase from the citric-acid-accumulating fungus Aspergillus niger (1992), Appl. Microbiol. Biotechnol., 37, 784-788.
No PubMed abstract available
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General Stability

General Stability Organism
very unstable upon purification Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
AMP weak Aspergillus niger
citrate weak Aspergillus niger
diphosphate not phosphate Aspergillus niger
additional information no inhibition by protein kinase C; phosphorylation by cAMP-dependent protein kinase, but no inhibition Aspergillus niger
phosphoenolpyruvate weak Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 beta-D-fructose 6-phosphate
-
 Aspergillus niger
0.7
-
 ATP
-
 Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Aspergillus niger
phosphate requirement Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation by cAMP-dependent protein kinase, but no inhibition Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus niger

Storage Stability

Storage Stability Organism
4°C, t1/2: 4 h Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Aspergillus niger ADP + beta-D-fructose 2,6-bisphosphate
-
 ?