Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxo-4-methylthiobutyrate | 1 mM, 10% inhibition | Lupinus luteus | |
5-Isobutylthioribose | competitive vs. 1-phospho-5-methylthioribose | Lupinus luteus | |
adenine | 1 mM, 25% inhibition | Lupinus luteus | |
ADP | 1 mM, 66% inhibition | Lupinus luteus | |
p-chloromercuribenzoate | - |
Lupinus luteus | |
S-adenosylhomocysteine | 1 mM, 36% inhibition | Lupinus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
5-methylthioribose | pH 9.7, 35°C | Lupinus luteus | |
0.0083 | - |
ATP | pH 9.7, 35°C | Lupinus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent metal ion is strictely required for activity, Mg2+ is most effective | Lupinus luteus | |
Mn2+ | divalent cation is strictley required for activity | Lupinus luteus | |
Mn2+ | 12% of activity with Mg2+ | Lupinus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Lupinus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-methylthioribose | Lupinus luteus | enzyme may be involved in an alternative pathway of methionine synthesis in plant tissues | ADP + 5-methylthioribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus luteus | - |
yellow lupin | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, aminohexyl-Sepharose, Sephadex g-200, hydroxyapatite Bio-Gel | Lupinus luteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Lupinus luteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1425 | - |
- |
Lupinus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alphaATP + 5-methylthioribose | - |
Lupinus luteus | alphaADP + 5-methylthioribose 1-phosphate | - |
? | |
ATP + 5-isobutylthioribose | - |
Lupinus luteus | ADP + 5-isobutylthioribose 1-phosphate | - |
? | |
ATP + 5-methylthioribose | no activity with AMP, ADP and alpha,beta-CH2-ATP | Lupinus luteus | ADP + 5-methylthioribose 1-phosphate | - |
? | |
ATP + 5-methylthioribose | enzyme may be involved in an alternative pathway of methionine synthesis in plant tissues | Lupinus luteus | ADP + 5-methylthioribose 1-phosphate | - |
? | |
dATP + 5-methylthioribose | 120% of activity with ATP | Lupinus luteus | dADP + 5-methylthioribose 1-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Lupinus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.5 | - |
Lupinus luteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | approx. 25% of maximal activity at pH 7.0 | Lupinus luteus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
5-Isobutylthioribose | - |
Lupinus luteus |