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Literature summary for 2.7.1.1 extracted from

  • Childers, C.L.; Storey, K.B.
    Post-translational regulation of hexokinase function and protein stability in the aestivating frog Xenopus laevis (2016), Protein J., 35, 61-71.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Urea
-
Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
D-glucose pH 8.0, 22°C Xenopus laevis
1.83
-
D-glucose enzyme isolated from dehydrated frogs, pH 8.0, 22°C Xenopus laevis
4.14
-
ATP pH 8.0, 22°C Xenopus laevis
4.21
-
ATP enzyme isolated from dehydrated frogs, pH 8.0, 22°C Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
x * 100000, SDS-PAGE Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from muscle Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
112
-
pH 8.0, 22°C Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
Xenopus laevis ADP + D-glucose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Xenopus laevis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54.2
-
melting temperature, enzyme isolated from dehydrated frogs Xenopus laevis
61.9
-
melting temperature Xenopus laevis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1230
-
pH 8.0, 22°C Xenopus laevis Urea
1380
-
enzyme isolated from dehydrated frogs, pH 8.0, 22°C Xenopus laevis Urea

General Information

General Information Comment Organism
physiological function hexokinase from the muscle of dehydrated frogs shows a 3.4 fold higher Vmax and 2.4fold higher Km for glucose compared with control, while the Km for ATP is unaltered. Hexokinase from dehydrated frogs also shows greater phosphoserine content (20% increase) and lower phosphothreonine (22% decrease) content compared to control. Control hexokinase has a higher melting temperature than from dehydrated frogs Xenopus laevis