Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | - |
Xenopus laevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
D-glucose | pH 8.0, 22°C | Xenopus laevis | |
1.83 | - |
D-glucose | enzyme isolated from dehydrated frogs, pH 8.0, 22°C | Xenopus laevis | |
4.14 | - |
ATP | pH 8.0, 22°C | Xenopus laevis | |
4.21 | - |
ATP | enzyme isolated from dehydrated frogs, pH 8.0, 22°C | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, SDS-PAGE | Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from muscle | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
112 | - |
pH 8.0, 22°C | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Xenopus laevis | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Xenopus laevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54.2 | - |
melting temperature, enzyme isolated from dehydrated frogs | Xenopus laevis |
61.9 | - |
melting temperature | Xenopus laevis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1230 | - |
pH 8.0, 22°C | Xenopus laevis | Urea | |
1380 | - |
enzyme isolated from dehydrated frogs, pH 8.0, 22°C | Xenopus laevis | Urea |
General Information | Comment | Organism |
---|---|---|
physiological function | hexokinase from the muscle of dehydrated frogs shows a 3.4 fold higher Vmax and 2.4fold higher Km for glucose compared with control, while the Km for ATP is unaltered. Hexokinase from dehydrated frogs also shows greater phosphoserine content (20% increase) and lower phosphothreonine (22% decrease) content compared to control. Control hexokinase has a higher melting temperature than from dehydrated frogs | Xenopus laevis |