Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.1 extracted from

  • Larion, M.; Salinas, R.; Bruschweiler-Li, L.; Miller, B.; Brüschweiler, R.
    Order-disorder transitions govern kinetic cooperativity and allostery of monomeric human glucokinase (2012), PLoS Biol., 10, e1001452.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + D-glucose = ADP + D-glucose 6-phosphate cutting-edge high-resolution nuclear magnetic resonance methods are used to explore the kinetic properties of glucokinase. Glucokinase samples a range of conformational states in the absence of glucose. In the presence of glucose or a small-molecule activator, the enzyme population shifts towards a more narrow, well-structured ensemble of states Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GCK
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens