Literature summary for 2.7.1.1 extracted from

Molnes, J.; Bjorkhaug, L.; Sovik, O.; Njolstad, P.R.; Flatmark, T.
Catalytic activation of human glucokinase by substrate binding: residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions (2008), FEBS J., 275, 2467-2481.

Search for more literature for 2.7.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
2-amino-4-fluoro-5-(1-methyl-1H-imidazol-2-ylsulfanyl)-N-thiazol-2-yl-benzamide	-	Homo sapiens
alpha-D-glucose	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D205A	the mutation in the L-domain results in enzyme form that does not bind alpha-D-glucose at 200 mM and is essentially catalytically inactive	Homo sapiens
E256A	the mutation in the L-domain results in enzyme form that does not bind alpha-D-glucose at 200 mM and is essentially catalytically inactive (2.6% relative activity compared to the wild type enzyme)	Homo sapiens
E256K	the mutation in the L-domain results in enzyme form that does not bind alpha-D-glucose at 200 mM and is essentially catalytically inactive	Homo sapiens
E290A	the mutant 45.9% relative activity compared to the wild type enzyme	Homo sapiens
K169N	the mutant shows 0.03% relative activity compared to the wild type enzyme	Homo sapiens
N204A	the mutation in the L-domain results in enzyme form that does not bind alpha-D-glucose at 200 mM and is essentially catalytically inactive (8.9% relative activity compared to the wild type enzyme)	Homo sapiens
N231A	the mutant shows 0.03% relative activity compared to the wild type enzyme	Homo sapiens
Q287V	the mutant 57.9% relative activity compared to the wild type enzyme	Homo sapiens
T168G	the mutant shows 9.4% relative activity compared to the wild type enzyme	Homo sapiens
W167F	the mutant exhibits 24fold reduction in catalytic efficiency	Homo sapiens
W257F	the mutant exhibits slightly reduced affinity for D-glucose	Homo sapiens
W99F	the mutant shows a small increase in both affinity and catalytic efficiency	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35557	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose column chromatography, Sephadex G-25 gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreatic beta cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-glucose	-	Homo sapiens	ADP + D-glucose 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
ATP: D-hexose 6-phosphotransferase	-	Homo sapiens
glucokinase	-	Homo sapiens
hGK isoform 1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.4	-	D-glucose	mutant enzyme W167F, in 25 mM HEPES (pH 7.4), at 37°C	Homo sapiens
42.1	-	D-glucose	mutant enzyme W257F, in 25 mM HEPES (pH 7.4), at 37°C	Homo sapiens
46.8	-	D-glucose	mutant enzyme W99F, in 25 mM HEPES (pH 7.4), at 37°C	Homo sapiens
60.4	-	D-glucose	wild type enzyme, in 25 mM HEPES (pH 7.4), at 37°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)