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Literature summary for 2.7.1.1 extracted from

  • Pedelini, L.; Garcia-Gimeno, M.A.; Marina, A.; Gomez-Zumaquero, J.M.; Rodriguez-Bada, P.; Lopez-Enriquez, S.; Soriguer, F.C.; Cuesta-Munoz, A.L.; Sanz, P.
    Structure-function analysis of the alpha5 and the alpha13 helices of human glucokinase: description of two novel activating mutations (2005), Protein Sci., 14, 2080-2086.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
A208G mutant displays a slightly higher glucose affinity and similar ATP affinity to the wild type enzyme. The activity of the enzyme at 5 mM glucose is moderately higher than the wild type activity Homo sapiens
A460R mutation increases the affinity of the enzyme for glucose Homo sapiens
C457V the mutant enzyme shows a slightly higher glucose level than the wild type and a slightly lower affinity for ATP. The activity of the enzymes at 5 mM glucose is higher than the wild type activity Homo sapiens
K458R mutation results in an enzyme with lower glucose affinity and lower ATP affinity than the wild type enzyme. Activity of the K458R mutant at 5 mM glucose is lower than the wild type activity Homo sapiens
K459L the mutant enzyme shows a slightly higher glucose than the wild type and a slightly lower affinity for ATP. The activity of the enzymes at 5 mM glucose is higher than the wild type activity Homo sapiens
Y215A mutation increases the affinity of the enzyme for glucose Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 ATP 30°C, mutant enzyme A456V Homo sapiens
0.29
-
 ATP 30°C, mutant enzyme >214C Homo sapiens
0.41
-
 ATP 30°C, wild-type enzyme Homo sapiens
0.43
-
 ATP 30°C, mutant enzyme A208G Homo sapiens
0.55
-
 ATP 30°C, mutant enzyme A460R Homo sapiens
0.6
-
 ATP 30°C, mutant enzyme K459L Homo sapiens
0.65
-
 ATP 30°C, mutant enzyme C457V Homo sapiens
0.65
-
 ATP 30°C, mutant enzyme Y215A Homo sapiens
0.81
-
 ATP 30°C, mutant enzyme K458R Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?