Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A464P/E465G | mutant with a helix breaker embedded in the interdomain alpha-helix has a smaller magnitude of phosphate alleviation than the wild type | Homo sapiens |
Q466_H467insHMNLAEQ | mutant has a smaller magnitude of phosphate alleviation than the wild type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-glucitol 6-phosphate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
D-glucose | pH 8.0, mutant enzyme A464P/E465G | Homo sapiens | |
0.0732 | - |
D-glucose | pH 8.0, wild-type enzyme | Homo sapiens | |
0.0841 | - |
D-glucose | pH 8.0, mutant enzyme Q466_H467insHMNLAEQ | Homo sapiens | |
1.09 | - |
ATP | pH 8.0, wild-type enzyme | Homo sapiens | |
1.16 | - |
ATP | pH 8.0, mutant enzyme A464P/E465G | Homo sapiens | |
1.51 | - |
ATP | pH 8.0, mutant enzyme Q466_H467insHMNLAEQ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0107 | - |
1,5-Anhydro-D-glucitol 6-phosphate | pH 8.0, mutant enzyme A464P/E465G | Homo sapiens | |
0.0108 | - |
1,5-Anhydro-D-glucitol 6-phosphate | pH 8.0, mutant enzyme Q466_H467insHMNLAEQ | Homo sapiens | |
0.0164 | - |
1,5-Anhydro-D-glucitol 6-phosphate | pH 8.0, wild-type enzyme | Homo sapiens |