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Literature summary for 2.7.1.1 extracted from
- The unique hexokinase of Kluyveromyces lactis. Molecular and functional characterization and evaluation of a role in glucose signaling (2003), J. Biol. Chem., 278, 39280-39286.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RAG5, overexpression in enzyme-deficient strain JA6DELTArag5 | Kluyveromyces lactis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inactivation by autophosphorylation at Ser157 utilizing MgATP2-, first order kinetics reaveal an extrapolated residual activity of about 60% | Kluyveromyces lactis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | stopped-flow steady-state kinetics, dissociation constants of the monomer-homodimer equilibria | Kluyveromyces lactis | |
| 0.196 | - |
D-glucose | pH 7.4, 25°C, recombinant enzyme at 0.001 mg/ml | Kluyveromyces lactis |  |
| 0.226 | - |
ATP | pH 7.4, 25°C, recombinant enzyme at 0.001 mg/ml | Kluyveromyces lactis | |
| 0.39 | - |
ATP | pH 7.4, 25°C, recombinant enzyme at 1.0 mg/ml | Kluyveromyces lactis | |
| 0.451 | - |
D-glucose | pH 7.4, 25°C, recombinant enzyme at 1.0 mg/ml | Kluyveromyces lactis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Kluyveromyces lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53476 | - |
1 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 1 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium | Kluyveromyces lactis |
| 53476 | - |
2 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 2 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium | Kluyveromyces lactis |
| 54000 | - |
1 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 1 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium | Kluyveromyces lactis |
| 54000 | - |
2 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 2 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium | Kluyveromyces lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Kluyveromyces lactis | - |
ADP + D-glucose 6-phosphate | - |
? | |
| additional information | Kluyveromyces lactis | the enzyme is the only glucose and fructose phosphorylating enzyme, which is upregulating glucose transport and also mediating glucose repression | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces lactis | P33284 | crabtree-negative yeast, gene RAG5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from enzyme-deficient strain JA6DELTArag5, 25fold by anion exchange chromatography and ammonium sulfate fractionation | Kluyveromyces lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Kluyveromyces lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Kluyveromyces lactis | ADP + D-glucose 6-phosphate | - |
? | |
| additional information | the enzyme is the only glucose and fructose phosphorylating enzyme, which is upregulating glucose transport and also mediating glucose repression | Kluyveromyces lactis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 2 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium | Kluyveromyces lactis |
| monomer | 1 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 1 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium | Kluyveromyces lactis |
| More | dissociation constants of the monomer-homodimer equilibria, hydrodynamic | Kluyveromyces lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KlHxk1 | - |
Kluyveromyces lactis |
| Rag5p | - |
Kluyveromyces lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Kluyveromyces lactis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Kluyveromyces lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Kluyveromyces lactis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Kluyveromyces lactis | |
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Release 2023.1 (January 2023)
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