Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N166R | increased affinity for glucose and ATP by a factor of 3 | Homo sapiens |
N166R-S151-A | lowers the KM-value for glucose by a factor of 40 and increases the KM-value for ATP | Homo sapiens |
S151A | lowers the KM-value for glucose by a factor of 26, increases the KM-value for ATP and decreases the KM-value for mannose and fructose | Homo sapiens |
S151C | lowers the KM-value for glucose by a factor of 2, increases the KM-value for ATP and decreases the KM-value for mannose and fructose | Homo sapiens |
S151G | lowers the KM-value for glucose by a factor of 40, increases the KM-value for ATP and decreases the KM-value for mannose and fructose | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | - |
Homo sapiens | |
N-acetylglucosamine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
D-mannose | 30°C, pH 7.5, N166R-S151A | Homo sapiens | |
0.12 | - |
D-mannose | 30°C, pH 7.5, S151G | Homo sapiens | |
0.15 | - |
D-glucose | 30°C, pH 7.5, S151G and N1166R-S151A | Homo sapiens | |
0.21 | - |
D-mannose | 30°C, pH 7.5, S151A | Homo sapiens | |
0.27 | - |
ATP | 30°C, pH 7.5, N166R | Homo sapiens | |
0.34 | - |
D-glucose | 30°C, pH 7.5, S151A | Homo sapiens | |
0.63 | - |
ATP | 30°C, pH 7.5, wild-type | Homo sapiens | |
1.52 | - |
ATP | 30°C, pH 7.5, N166R-S151A | Homo sapiens | |
1.9 | - |
2-deoxy-D-glucose | 30°C, pH 7.5, S151A | Homo sapiens | |
2.1 | - |
D-glucose | 30°C, pH 7.5, S151C and N166R | Homo sapiens | |
2.2 | - |
D-mannose | 30°C, pH 7.5, N166R | Homo sapiens | |
2.21 | - |
ATP | 30°C, pH 7.5, S151A | Homo sapiens | |
2.26 | - |
ATP | 30°C, pH 7.5, S151G | Homo sapiens | |
2.6 | - |
ATP | 30°C, pH 7.5, S151C | Homo sapiens | |
3.74 | - |
D-mannose | 30°C, pH 7.5, S151C | Homo sapiens | |
4.4 | - |
D-mannose | 30°C, pH 7.5, wild-type | Homo sapiens | |
4.7 | - |
D-fructose | 30°C, pH 7.5, N166R-S151A | Homo sapiens | |
6 | - |
D-glucose | 30°C, pH 7.5, wild-type | Homo sapiens | |
7.7 | - |
D-fructose | 30°C, pH 7.5, S151G | Homo sapiens | |
18 | - |
2-deoxyglucose | 30°C, pH 7.5, wild-type | Homo sapiens | |
57.3 | - |
D-fructose | 30°C, pH 7.5, S151A | Homo sapiens | |
77 | - |
D-fructose | 30°C, pH 7.5, N166R | Homo sapiens | |
240 | - |
D-fructose | 30°C, pH 7.5, wild-type and S151C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Homo sapiens | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
pancreatic islet | beta-cells | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
S151G, D-mannose as substrate | Homo sapiens |
0.6 | - |
S151G, D-glucose as substrate | Homo sapiens |
1.4 | - |
N166R-S151A, D-mannose as substrate | Homo sapiens |
2 | - |
N166R-S151A, D-glucose as substrate | Homo sapiens |
2.16 | - |
S151C, D-mannose as substrate | Homo sapiens |
3.18 | - |
S151G, D-fructose as substrate | Homo sapiens |
3.32 | - |
S151A, D-mannose as substrate | Homo sapiens |
4.1 | - |
S151A, 2-deoxy-D-glucose as substrate | Homo sapiens |
6 | - |
S151C, D-glucose as substrate | Homo sapiens |
6.6 | - |
S151A, D-glucose as substrate | Homo sapiens |
9.12 | - |
S151C, D-fructose as substrate | Homo sapiens |
14.5 | - |
N166R-S151A, D-fructose as substrate | Homo sapiens |
23.8 | - |
S151A, D-fructose as substrate | Homo sapiens |
60.8 | - |
N166R, D-mannose as substrate | Homo sapiens |
68 | - |
wild-type, D-mannose and 2-deoxy-D-glucose as substrate | Homo sapiens |
80 | - |
wild-type and N166R, D-glucose as substrate | Homo sapiens |
122 | - |
wild-type, D-fructose as substrate | Homo sapiens |
310 | - |
N166R, D-fructose as substrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | - |
Homo sapiens | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + D-fructose | - |
Homo sapiens | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | - |
Homo sapiens | ADP + D-mannose 6-phosphate | - |
? |