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Literature summary for 2.7.1.1 extracted from
- Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein (1996), Mol. Cell. Biochem., 155, 23-29.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| carboxyl-domain of hexokinase type III is cloned, sequenced and overexpressed in Escherichia coli XL1-Blue as a glutathione S-transferase fusion protein, recombinant protein is catalytically active | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | hexokinase type III, but not its catalytically active recombinant carboxyl-domain, at concentrations above 0.5 mM | Homo sapiens |  |
| D-glucose 6-phosphate | hexokinase type III and its catalytically active recombinant carboxyl-domain | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
D-glucose | hexokinase III from lymphocytes, at 37°C, pH 8.1 | Homo sapiens | |
| 0.038 | - |
D-glucose | catalytically active recombinant carboxyl-domain of hexokinase III, at 37°C, pH 8.1 | Homo sapiens | |
| 1.25 | - |
ATP | hexokinase III from lymphocytes, at 37°C, pH 8.1 | Homo sapiens | |
| 3.05 | - |
ATP | catalytically active recombinant carboxyl-domain of hexokinase III, at 37°C, pH 8.1 | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, catalytically active recombinant carboxyl-domain of hexokinase type III, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
hexokinase type III and its catalytically active recombinant carboxyl-domain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 120fold: recombinant carboxyl-domain of hexokinase type III, partial: hexokinase type III from lymphocytes | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | carboxyl-domain of hexokinase type III | Homo sapiens | - |
| lymphocyte | hexokinase type III | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36 | - |
catalytically active recombinant carboxyl-domain of hexokinase type III, at 37°C, pH 8.1 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ATP | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
| additional information | role of the hexokinase III carboxyl-domain in determining the catalytic properties of enzyme | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, catalytically active recombinant carboxyl-domain of hexokinase type III, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
D-glucose 6-phosphate | catalytically active recombinant carboxyl-domain of hexokinase III, at 37°C, pH 7.2 | Homo sapiens | |
| 0.226 | - |
D-glucose 6-phosphate | hexokinase III from lymphocytes, at 37°C, pH 7.2 | Homo sapiens | |
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