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Literature summary for 2.7.1.1 extracted from

  • Aleshin, A.E.; Zeng, C.; Bourenkov, G.P.; Bartunik, H.D.; Fromm, H.J.; Honzatko, R.B.
    The mechanism of regulation of hexokinase: New insights from the crystal structure of recombinant human brain hexokinase complexed with glucose and glucose-6-phosphate (1998), Structure, 6, 39-50.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding hexokinase I is cloned and expressed in Escherichia coli BL21(DE3), amino acid sequence Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of hexokinase I from brain complexed with glucose and glucose 6-phosphate Homo sapiens
hanging drop method Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-phosphate potent inhibition of hexokinase I is relieved by physiological levels of phosphate, mechanism Homo sapiens
N-acetyl-D-glucosamine potent inhibitor of hexokinase I Homo sapiens
N-methylglucosamine potent inhibitor of hexokinase I Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 2 * 100000 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Homo sapiens hexokinase I is the pacemaker of glycolysis in brain tissue D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 recombinant hexokinase I, expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant hexokinase I expressed in Escherichia coli BL21(DE3) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain hexokinase I Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosamine + ATP
-
 Homo sapiens ADP + D-glucosamine 6-phosphate
-
 ?
D-glucose + ATP bound glucose is present as the beta-anomer, glucose binding sites Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP hexokinase I is the pacemaker of glycolysis in brain tissue Homo sapiens D-glucose 6-phosphate + ADP
-
 ?
additional information C-terminal catalytic and N-terminal regulatory domain, detailed structure of hexokinase I, substrate binding sites, models for regulation Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 100000 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens