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Literature summary for 2.7.1.1 extracted from

  • Magnani, M.; Serafini, G.; Stocchi, V.; Bossu, M.; Dacha, M.
    Solubilization, purification, and properties of rabbit brain hexokinase (1982), Arch. Biochem. Biophys., 216, 449-454.
    View publication on PubMed
Search for more literature for 2.7.1.1

General Stability

General Stability Organism
D-glucose or D-fructose, 5 mM, stabilizes Oryctolagus cuniculus
glycerol, 9-15% v/v, stabilizes Oryctolagus cuniculus
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
0.1
-
 D-mannose at 37°C, pH 8.1 Oryctolagus cuniculus
0.4
-
 D-glucosamine at 37°C, pH 8.1 Oryctolagus cuniculus
0.62
-
 ATP MgATP2-, at 37°C, pH 8.1 Oryctolagus cuniculus
2
-
 2-deoxy-D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
4.5
-
 D-fructose at 37°C, pH 8.1 Oryctolagus cuniculus
10
-
 ITP MgITP2-, at 37°C, pH 8.1 Oryctolagus cuniculus
30
-
 UTP MgUTP2-, at 37°C, pH 8.1 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Oryctolagus cuniculus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 sucrose density gradient sedimentation Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
717fold Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
165
-
 at 37°C Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
4°C or -20°C, 5 mM sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, 3 mM mercaptoethanol, several days, stable Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose 54% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
ATP + 2-deoxy-D-glucose 84% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
D-fructose + ATP 110% of the activity with glucose Oryctolagus cuniculus ADP + D-fructose 6-phosphate
-
 ?
D-glucosamine + ATP
-
 Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate
-
 ?
D-glucose + ATP
-
 Oryctolagus cuniculus ADP + D-glucose 6-phosphate
-
 ?
D-mannose + ATP 82% of the activity with glucose Oryctolagus cuniculus ADP + D-mannose 6-phosphate
-
 ?
ITP + D-glucose 12% of the activity with ATP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose MgITP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
additional information not: N-acetyl-D-glucosamine Oryctolagus cuniculus ?
-
 ?
additional information not: D-galactose Oryctolagus cuniculus ?
-
 ?
additional information not: CTP Oryctolagus cuniculus ?
-
 ?
UTP + D-glucose 14% of the activity with ATP Oryctolagus cuniculus UDP + D-glucose 6-phosphate
-
 ?
UTP + D-glucose MgUTP Oryctolagus cuniculus UDP + D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 in glycyl-glycine buffer Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Oryctolagus cuniculus

pI Value

Organism Comment pI Value Maximum pI Value
Oryctolagus cuniculus isoelectric focusing 6.8 6.3