General Stability | Organism |
---|---|
D-glucose, D-fructose, D-mannose and high concentrations of glycerol, 10-15% v/v, stabilize the purified enzyme | Sus scrofa |
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | higher than 0.4 mM; inhibits hexokinase III | Sus scrofa | |
D-glucose 6-phosphate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
D-mannose | hexokinase III, at 30°C, pH 8.1 | Sus scrofa | |
0.015 | - |
D-glucose | hexokinase III, at 30°C, pH 8.1 | Sus scrofa | |
0.033 | - |
2-deoxy-D-glucose | hexokinase III, at 30°C, pH 8.1 | Sus scrofa | |
0.15 | - |
D-glucose | hexokinase II, at 30°C, pH 8.1 | Sus scrofa | |
0.2 | - |
D-glucosamine | hexokinase III, at 30°C, pH 8.1 | Sus scrofa | |
0.3 | - |
ATP | hexokinase II, at 30°C, pH 8.1 | Sus scrofa | |
0.5 | - |
ATP | hexokinase III, at 30°C, pH 8.1 | Sus scrofa | |
1.5 | - |
D-fructose | hexokinase III, at 30°C, pH 8.1 | Sus scrofa | |
2.5 | - |
ITP | hexokinase III, at 30°C, pH 8.1 | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Sus scrofa |
100000 | - |
hexokinases II and III | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
adult pigs | - |
Sus scrofa | - |
2 enzyme forms: hexokinase type II and type III | - |
Purification (Comment) | Organism |
---|---|
hexokinase III: 290000fold, hexokinase II: partial, 39160fold | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | hexokinase types II and III, hexokinase III is the predominant form in adult pig erythrocytes, hexokinase II in newborn pig erythrocytes | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
hexokinase type II, at 37°C, pH 8.1 | Sus scrofa |
191.5 | - |
hexokinase type III, at 37°C, pH 8.1 | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | hexokinase III: 151% of the activity with glucose | Sus scrofa | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
CTP + D-glucose | - |
Sus scrofa | CDP + D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | hexokinase III: 12.6% of the activity with glucose | Sus scrofa | ADP + D-fructose 6-phosphate | - |
? | |
D-glucosamine + ATP | hexokinase III: 35% of the activity with glucose | Sus scrofa | ADP + D-glucosamine 6-phosphate | - |
? | |
D-glucose + ATP | - |
Sus scrofa | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | hexokinase III: 85% of the activity with glucose | Sus scrofa | ADP + D-mannose 6-phosphate | - |
? | |
ITP + D-glucose | hexokinase III: 24% of the activity with glucose | Sus scrofa | IDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | MgITP | Sus scrofa | IDP + D-glucose 6-phosphate | - |
? | |
additional information | not: UTP | Sus scrofa | ? | - |
? | |
additional information | not: GTP | Sus scrofa | ? | - |
? | |
additional information | not: CTP | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, hexokinase III, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
5 mM sodium phosphate buffer, pH 7.5, 0.25 mM glucose, 10% v/v glycerol, 1 mM dithiothreitol, inactivation as a function of time, hexokinase type III is more stable than hexokinase type II | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
hexokinase type III | Sus scrofa |
9 | - |
hexokinase type II | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sus scrofa | hexokinase III, isoelectric focusing | 4.4 | 4.3 |
Sus scrofa | hexokinase II, isoelectric focusing | - |
4.8 |