General Stability | Organism |
---|---|
storage of purified enzyme in D-glucose-containing phosphate buffer enhances its stability | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-glucitol 6-phosphate | intact enzyme and catalytically active 51 kDa C fragment of hexokinase | Rattus norvegicus | |
arsenate | catalytically active 51 kDa C fragment of hexokinase | Rattus norvegicus | |
D-glucose 6-phosphate | - |
Rattus norvegicus | |
phosphate | competitive versus ATP; intact enzyme: at high concentrations, catalytically active 51 kDa C fragment of hexokinase: also at low concentrations; noncompetitive to glucose | Rattus norvegicus | |
SO42- | catalytically active 51 kDa C fragment of hexokinase | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
retention of the full catalytic activity of rat brain hexokinase C-terminal half supports the suggestion that the 100000 MW hexokinase evolves from an ancestral 50000 MW yeast type hexokinase by a process of gene duplication | Rattus norvegicus |
100000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Rattus norvegicus | initial catalytic step of the cerebral glucose metabolism, glucose 6-phosphate represents a major regulatory influence on the in vivo activity | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
hexokinase type I | - |
Purification (Comment) | Organism |
---|---|
intact hexokinase I, isolation of a catalytically active 51 kDa C fragment and a 52 kDa N fragment without catalytic activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | hexokinase I | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60 | - |
intact 100 kDa enzyme | Rattus norvegicus |
110 | - |
catalytically active 51 kDa C fragment of hexokinase | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + ATP | poor substrate | Rattus norvegicus | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | good substrate | Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | initial catalytic step of the cerebral glucose metabolism, glucose 6-phosphate represents a major regulatory influence on the in vivo activity | Rattus norvegicus | D-glucose 6-phosphate + ADP | - |
? | |
D-mannose + ATP | good substrate | Rattus norvegicus | ADP + D-mannose 6-phosphate | - |
? | |
additional information | isolated catalytically active 51 kDa C-fragment and 52 kDa N-fragment without catalytic activity, both fragments contain discrete binding sites for hexoses and hexose 6-phosphates, one of each pair of sites must be latent in the intact enzyme, the regulatory site binding glucose 6-phosphate is associated with the N-terminal half | Rattus norvegicus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 0.02 | 1,5-Anhydro-D-glucitol 6-phosphate | catalytically active 51 kDa C fragment of hexokinase | Rattus norvegicus | |
6 | - |
phosphate | catalytically active 51 kDa C fragment of hexokinase, versus ATP | Rattus norvegicus | |
35 | - |
phosphate | intact hexokinase I | Rattus norvegicus | |
60 | - |
phosphate | catalytically active 51 kDa C fragment of hexokinase, versus glucose | Rattus norvegicus |