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Literature summary for 2.7.1.1 extracted from
- Purification of yeast hexokinase isoenzymes using affinity chromatography and chromatofocusing (1982), Anal. Biochem., 121, 181-185.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | uncomplexed | Saccharomyces cerevisiae |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
haploid strain cat1.S3-14A of genotype a his MAL2-8c MAL3 SUC3, isoenzymes PI and PII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Saccharomyces cerevisiae |
| isoenzymes PI and PII | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, polybuffer, over 8 days, stable | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + ATP | - |
Saccharomyces cerevisiae | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucose + ATP | - |
Saccharomyces cerevisiae | ADP + D-glucose 6-phosphate | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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