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Literature summary for 2.7.1.1 extracted from

  • Machado de Domenech, E.E.; Sols, A.
    Specificity of hexokinases towards some uncommon substrates and inhibitors (1980), FEBS Lett., 119, 174-176.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-thio-D-glucose
-
Bos taurus
6-deoxy-D-glucose
-
Saccharomyces cerevisiae
N-acetylmannosamine
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
2-deoxy-2-fluoro-D-glucose at 37°C, pH 7.4 Saccharomyces cerevisiae
0.2
-
2-deoxy-2-fluoro-D-glucose at 37°C, pH 7.4 Bos taurus
4
-
5-thio-D-glucose
-
Saccharomyces cerevisiae
5
-
D-mannosamine at 37°C, pH 7.4 Saccharomyces cerevisiae
5
-
1-thio-D-glucose at 37°C, pH 7.4 Saccharomyces cerevisiae
20
-
1,5-anhydro-D-glucitol at 37°C, pH 7.4 Saccharomyces cerevisiae
30
-
3-deoxy-3-amino-D-glucose at 37°C, pH 7.4 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Saccharomyces cerevisiae
-
isoenzymes I and II
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,5-anhydro-D-glucitol weak Saccharomyces cerevisiae ADP + 1,5-anhydro-D-glucitol 6-phosphate
-
?
ATP + 1-thio-D-glucose isoenzyme II, weak Saccharomyces cerevisiae ADP + 1-thio-D-glucose 6-phosphate
-
?
ATP + 2-deoxy-2-fluoro-D-glucose good substrate Saccharomyces cerevisiae ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate
-
?
ATP + 2-deoxy-2-fluoro-D-glucose good substrate Bos taurus ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate
-
?
ATP + 3-deoxy-3-amino-D-glucose isoenzyme II, weak Saccharomyces cerevisiae ADP + 3-deoxy-3-amino-D-glucose 6-phosphate
-
?
ATP + 5-thio-D-glucose very slow phosphorylation Saccharomyces cerevisiae ADP + 5-thio-D-glucose 6-phosphate
-
?
ATP + 5-thio-D-glucose very slow phosphorylation Bos taurus ADP + 5-thio-D-glucose 6-phosphate
-
?
ATP + D-mannosamine fairly good substrate Saccharomyces cerevisiae ADP + D-mannosamine 6-phosphate
-
?
ATP + D-mannosamine fairly good substrate Bos taurus ADP + D-mannosamine 6-phosphate
-
?
CTP + D-glucose 35% of the activity with ATP Saccharomyces cerevisiae CDP + D-glucose 6-phosphate
-
?
D-glucose + ATP
-
Saccharomyces cerevisiae ADP + D-glucose 6-phosphate
-
?
D-glucose + ATP
-
Bos taurus ADP + D-glucose 6-phosphate
-
?
additional information not: N-acetylmannosamine, 6-deoxy-6-aminoglucose Saccharomyces cerevisiae ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae
ATP
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
5-thio-D-glucose inhibition of fructose phosphorylation, at 37°C, pH 7.4 Bos taurus
50
-
6-deoxy-D-glucose at 37°C, pH 7.4 Saccharomyces cerevisiae
50
-
N-acetylmannosamine at 37°C, pH 7.4 Saccharomyces cerevisiae