General Stability | Organism |
---|---|
D-glucose or D-fructose, 5 mM, stabilizes | Oryctolagus cuniculus |
glycerol, 9-15% v/v, stabilizes | Oryctolagus cuniculus |
sulfhydryl protecting agents stabilize | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | uncomplexed, competitive to MgATP2-, erythrocyte enzyme | Oryctolagus cuniculus | |
D-glucose 6-phosphate | linear competitive inhibition with MgATP2- as varied substrate | Oryctolagus cuniculus | |
GSSG | strong inhibitor at all concentrations | Oryctolagus cuniculus | |
Mg2+ | competitive to MgATP2-; uncomplexed | Oryctolagus cuniculus | |
MgADP- | erythrocyte enzyme, mixed inhibitor versus MgATP2- and D-glucose | Oryctolagus cuniculus | |
N-acetyl-D-glucosamine | competitive to glucose; erythrocyte enzyme; noncompetitive to MgATP2- | Oryctolagus cuniculus | |
reduced glutathione | 1 mM: reduced state of the enzyme with full catalytic activity, marked inhibition at high concentrations | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-glucose | reticulocyte hexokinase Ia | Oryctolagus cuniculus | |
0.06 | - |
D-glucose | erythrocyte enzyme | Oryctolagus cuniculus | |
0.125 | - |
D-glucose | reticulocyte hexokinase Ib | Oryctolagus cuniculus | |
0.5 | - |
ATP | MgATP2- | Oryctolagus cuniculus | |
0.5 | - |
ATP | reticulocyte and erythrocyte enzymes | Oryctolagus cuniculus | |
0.6 | - |
ATP | MgATP2-, erythrocyte enzyme | Oryctolagus cuniculus | |
0.71 | - |
D-mannose | erythrocyte enzyme | Oryctolagus cuniculus | |
1.33 | - |
2-deoxy-D-glucose | erythrocyte enzyme | Oryctolagus cuniculus | |
1.9 | - |
ITP | MgITP2-, erythrocyte enzyme | Oryctolagus cuniculus | |
2 | - |
D-glucosamine | erythrocyte enzyme | Oryctolagus cuniculus | |
17.8 | - |
D-fructose | erythrocyte enzyme | Oryctolagus cuniculus | |
41.6 | - |
N-acetyl-D-glucosamine | erythrocyte enzyme | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE | Oryctolagus cuniculus |
105000 | - |
reticulocyte hexokinase Ia and Ib, gel filtration, sucrose density gradient sedimentation | Oryctolagus cuniculus |
110000 | - |
gel filtration | Oryctolagus cuniculus |
110000 | - |
erythrocyte enzyme | Oryctolagus cuniculus |
112000 | - |
erythrocyte enzyme, sedimentation velocity | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
erythrocyte enzyme and reticulocyte hexokinase Ia and Ib | Oryctolagus cuniculus |
erythrocyte enzyme: 300000fold | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-hexose = ADP + D-hexose 6-phosphate | rapid random kinetic mechanism, erythrocyte enzyme and hexokinase I | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | only one enzyme form | Oryctolagus cuniculus | - |
reticulocyte | hexokinase Ia and Ib | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
144 | - |
reticulocyte, hexokinase Ib, at 37°C | Oryctolagus cuniculus |
145.6 | - |
erythrocyte, at 37°C | Oryctolagus cuniculus |
155 | - |
reticulocyte, hexokinase Ia, at 37°C | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, 5 mM sodium-potassium phosphate buffer, pH 7.5, erythrocyte enzyme is more stable than in other buffer systems | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | 65.7% of the activity with glucose, erythrocyte enzyme | Oryctolagus cuniculus | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | 70% of the activity with glucose, erythrocyte enzyme | Oryctolagus cuniculus | ADP + D-fructose 6-phosphate | - |
? | |
D-glucosamine + ATP | erythrocyte enzyme, 43.4% of the activity with glucose | Oryctolagus cuniculus | ADP + D-glucosamine 6-phosphate | - |
? | |
D-glucose + ATP | MgATP2- | Oryctolagus cuniculus | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | 109% of the activity with glucose, erythrocyte enzyme | Oryctolagus cuniculus | ADP + D-mannose 6-phosphate | - |
? | |
ITP + D-glucose | 36% of the activity with ATP, erythrocyte enzyme | Oryctolagus cuniculus | IDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | MgITP | Oryctolagus cuniculus | IDP + D-glucose 6-phosphate | - |
? | |
additional information | not: D-galactose | Oryctolagus cuniculus | ? | - |
? | |
N-acetyl-D-glucosamine + ATP | erythrocyte enzyme, 32.6% of the activity with glucose | Oryctolagus cuniculus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 110000, erythrocyte enzyme, SDS-PAGE | Oryctolagus cuniculus |
monomer | 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
D-glucose 6-phosphate | - |
Oryctolagus cuniculus | |
0.7 | - |
N-acetyl-D-glucosamine | erythrocyte enzyme, competitive to glucose | Oryctolagus cuniculus | |
1.5 | - |
ATP | uncomplexed ATP, erythrocyte enzyme, competitive to MgATP2- | Oryctolagus cuniculus | |
3 | - |
MgADP- | erythrocyte enzyme, mixed inhibitor versus MgATP2- | Oryctolagus cuniculus | |
7.8 | - |
MgADP- | erythrocyte enzyme, mixed inhibitor versus glucose | Oryctolagus cuniculus | |
9 | - |
Mg2+ | erythrocyte enzyme, uncomplexed Mg2+ | Oryctolagus cuniculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryctolagus cuniculus | under native conditions | - |
5.7 |
Oryctolagus cuniculus | reticulocyte hexokinase Ib | - |
5.7 |
Oryctolagus cuniculus | erythrocyte enzyme and reticulocyte hexokinase Ia, under native conditions | - |
6.2 |