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Literature summary for 2.6.99.3 extracted from

  • Uda, N.; Matoba, Y.; Kumagai, T.; Oda, K.; Noda, M.; Sugiyama, M
    . Establishment of an in vitro D-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway (2013), Antimicrob. Agents Chemother., 57, 2603-2612.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.99.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9
-
 Hydroxyurea 30°C, pH 8.0 Streptomyces lavendulae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Streptomyces lavendulae
126000
-
 gel filtration Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-serine + hydroxyurea Streptomyces lavendulae the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species O-ureido-L-serine + acetate
-
 ?
O-acetyl-L-serine + hydroxyurea Streptomyces lavendulae ATCC 11924 the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species O-ureido-L-serine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae D2Z027
-
-
Streptomyces lavendulae ATCC 11924 D2Z027
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + hydroxyurea the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species Streptomyces lavendulae O-ureido-L-serine + acetate
-
 ?
O-acetyl-L-serine + hydroxyurea O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O3-acetyl-D-serine and L-serine. The enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme also catalyzes the reaction with O-acetyl-L-serine and hydrogen sulfide (with 80fold higher catalytic efficiency) Streptomyces lavendulae O-ureido-L-serine + acetate
-
 ?
O-acetyl-L-serine + hydroxyurea the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species Streptomyces lavendulae ATCC 11924 O-ureido-L-serine + acetate
-
 ?
O-acetyl-L-serine + hydroxyurea O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O3-acetyl-D-serine and L-serine. The enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme also catalyzes the reaction with O-acetyl-L-serine and hydrogen sulfide (with 80fold higher catalytic efficiency) Streptomyces lavendulae ATCC 11924 O-ureido-L-serine + acetate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Streptomyces lavendulae

Synonyms

Synonyms Comment Organism
DcsD
-
 Streptomyces lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 Hydroxyurea 30°C, pH 8.0 Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces lavendulae
9
-
 activity increases from pH 5.0 to pH 9.0 Streptomyces lavendulae

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 activity at pH 7.5 is about 45% compared to activity at pH 9.0 Streptomyces lavendulae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 O-acetyl-L-serine 30°C, pH 8.0, cosubstrate: hydroxyurea Streptomyces lavendulae
1.2
-
 Hydroxyurea 30°C, pH 8.0 Streptomyces lavendulae