BRENDA - Enzyme Database
show all sequences of 2.6.99.3

Molecular cloning and heterologous expression of a biosynthetic gene cluster for the antitubercular agent D-cycloserine produced by Streptomyces lavendulae

Kumagai, T.; Koyama, Y.; Oda, K.; Noda, M.; Matoba, Y.; Sugiyama, M.; Antimicrob. Agents Chemother. 54, 1132-1139 (2010)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae ATCC 11924
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces lavendulae
D2Z027
-
-
Streptomyces lavendulae ATCC 11924
D2Z027
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
DcsD
-
Streptomyces lavendulae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae ATCC 11924
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
Other publictions for EC 2.6.99.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737505
Kumagai
High-level heterologous produc ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
Appl. Environ. Microbiol.
81
7881-7887
2015
-
1
-
-
-
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-
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5
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4
-
1
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1
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4
-
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-
738218
Uda
The structural and mutational ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
FEBS J.
282
3929-3944
2015
-
-
1
1
6
-
-
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
4
1
1
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
1
6
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
2
-
-
-
-
-
-
-
-
6
6
721306
Uda
. Establishment of an in vitro ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
Antimicrob. Agents Chemother.
57
2603-2612
2013
-
-
1
-
-
-
-
1
-
-
2
2
-
6
-
-
1
-
-
-
-
-
4
1
1
1
-
-
1
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
-
-
-
4
1
1
-
-
1
2
1
-
-
-
-
-
-
2
2
721300
Kumagai
Molecular cloning and heterolo ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
Antimicrob. Agents Chemother.
54
1132-1139
2010
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2
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5
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4
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1
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2
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4
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