Literature summary for 2.6.99.2 extracted from

Garrido-Franco, M.; Laber, B.; Huber, R.; Clausen, T.
Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis (2002), J. Mol. Biol., 321, 601-612.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, the native enzyme crystals are incubated with 2.5 mM glyceraldehyde 3-phosphate and 2.5 mM 1-deoxy-D-xylulose 5-phosphate for 3 hours, in 10 mM or 100 mM phosphate for 3 days, and for another 3 days in 20 mM 1-deoxy-D-xylulose 5-phosphate, X-ray diffraction structure determination and analysis at 2.3 A resolution	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate	Escherichia coli	-	pyridoxine 5'-phosphate + phosphate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A794	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O	reaction mechanism, active site structure, substrate binding structure, structure-function relationship	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate	-	Escherichia coli	pyridoxine 5'-phosphate + phosphate + H2O	-	?
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate	1-deoxy-D-xylose 5-phosphate binding structure	Escherichia coli	pyridoxine 5'-phosphate + phosphate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
PdxJ	-	Escherichia coli
PNP synthase	-	Escherichia coli

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Release 2023.1 (January 2023)