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Literature summary for 2.6.1.B21 extracted from

  • Chen, F.F.; Zhang, Y.H.; Zhang, Z.J.; Liu, L.; Wu, J.P.; Xu, J.H.; Zheng, G.W.
    An ammonium-formate-driven trienzymatic cascade for omega-transaminase-catalyzed (R)-selective amination (2019), J. Org. Chem., 84, 14987-14993 .
    View publication on PubMed
Search for more literature for 2.6.1.B21

Application

Application Comment Organism
synthesis development of a one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products Aspergillus terreus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli strain BL21(DE3) Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus Q0C8G1
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Aspergillus terreus FGSC A1156 Q0C8G1
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Aspergillus terreus NIH 2624 Q0C8G1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview Aspergillus terreus ?
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additional information (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview Aspergillus terreus NIH 2624 ?
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-
additional information (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview Aspergillus terreus FGSC A1156 ?
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-

Synonyms

Synonyms Comment Organism
(R)-amine:pyruvate transaminase
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 Aspergillus terreus
(R)-omega-TA
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 Aspergillus terreus
(R)-selective aminotransferase
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 Aspergillus terreus
(R)-selective omega-TA
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 Aspergillus terreus
At-omega-TA
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 Aspergillus terreus
ATEG_10023 locus name Aspergillus terreus
omega-transaminase
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 Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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 assay at Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
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 assay at Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
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 Aspergillus terreus