Application | Comment | Organism |
---|---|---|
synthesis | the covalently immobilized enzyme is used for amine bioproduction in batch and flow reaction systems, method evaluation, overview. Produced vanillylamine is a valuable building block for the synthesis of natural products, such as capsaicinoids | Halomonas elongata |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of an efficient bioproduction of amines by exploiting the immobilised (S)-selective amine transaminase from Halomonas elongata (HEWT), which shows a very broad substrate scope, tolerating a range of temperatures, pH values, salts, and co-solvents, in a continuous flow-reactor. The immobilised HEWT (imm-HEWT) is applied to continuous-flow reactions maintaining the biocatalyst in a packed-bed reactor to decrease reaction times and increase the productivity. The addition of an in-line purification step allows for the recovery of the pure products without any additional work-up procedure. Method optimization and evaluation, overview | Halomonas elongata |
General Stability | Organism |
---|---|
the HEWT quaternary structure is quite unstable | Halomonas elongata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas elongata | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-(-)-1-phenyl-ethylamine + 4-nitrobenzaldehyde | complete conversion at pH 8.0 and 37°C | Halomonas elongata | 4-nitrobenzylamine + L-alanine | - |
? | |
(S)-(-)-1-phenyl-ethylamine + cinnamaldehyde | - |
Halomonas elongata | cinnamylamine + L-alanine | - |
? | |
(S)-(-)-1-phenyl-ethylamine + vanillin | - |
Halomonas elongata | vanillylamine + L-alanine | - |
? | |
(S)-1-phenylethylamine + pyruvate | - |
Halomonas elongata | L-alanine + acetophenone | - |
r | |
additional information | vanillylamine is a valuable building block for the synthesis of natural products, such as capsaicinoids | Halomonas elongata | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
(S)-selective amine transaminase | - |
Halomonas elongata |
HEWT | - |
Halomonas elongata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Halomonas elongata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Halomonas elongata |
General Information | Comment | Organism |
---|---|---|
physiological function | transaminases play a central role in the biocatalytic preparation of enantiopure amines and amino acids, challenging reactions to achieve by conventional synthesis | Halomonas elongata |