Application | Comment | Organism |
---|---|---|
synthesis | amine transaminase (ATA) catalyzing stereoselective amination of prochiral ketones is an attractive alternative to transition metal catalysis | Ruegeria sp. TM1040 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutational analysis of enzyme-substrate interactions and substrate specificity, overview | Ruegeria sp. TM1040 |
P281S | site-directed mutagenesis, the mutation seems to have a negative effect on specific activity | Ruegeria sp. TM1040 |
Y152F | site-directed mutagenesis, the mutation stabilizes the enzyme, the activity is slightly reduced compared to wild-type | Ruegeria sp. TM1040 |
Y59W/T231A | site-directed mutagenesis, inactive mutant | Ruegeria sp. TM1040 |
Y59W/Y87F/T231A | site-directed mutagenesis, inactive mutant | Ruegeria sp. TM1040 |
Y59W/Y87L/T231A | site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type | Ruegeria sp. TM1040 |
Y59W/Y87L/T231A/G429A | site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type | Ruegeria sp. TM1040 |
Y59W/Y87L/T231A/L382M | site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type | Ruegeria sp. TM1040 |
Y59W/Y87L/T231A/L382M/G429A | site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type | Ruegeria sp. TM1040 |
Y59W/Y87L/Y152F/T231A/L382M/G429A | site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type | Ruegeria sp. TM1040 |
Y59W/Y87V/T231A | site-directed mutagenesis | Ruegeria sp. TM1040 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruegeria sp. TM1040 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0065 | - |
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87V/T231A | Ruegeria sp. TM1040 |
0.0115 | - |
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A | Ruegeria sp. TM1040 |
0.019 | - |
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/L382M | Ruegeria sp. TM1040 |
0.0324 | - |
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/P281S/G429A | Ruegeria sp. TM1040 |
0.0448 | - |
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/G429A | Ruegeria sp. TM1040 |
0.0771 | - |
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/L382M/G429A | Ruegeria sp. TM1040 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-dimethyl-1-phenylpropan-1-one + isopropylamine | the conversion by all active enzyme mutants lays between 22 and 71% with isopropylamine as amine donor, with enantiomeric excess above 99%, low activity | Ruegeria sp. TM1040 | (R)-2,2-dimethyl-1-phenylpropan-1-amine + acetone | - |
r | |
2,2-dimethyl-1-phenylpropan-1-one + L-alanine | the conversion by all active enzyme mutants is complete with L-alanine as amine donor, with enantiomeric excess above 99% | Ruegeria sp. TM1040 | (R)-2,2-dimethyl-1-phenylpropan-1-amine + pyruvate | - |
r | |
additional information | engineered ATAs perform asymmetric synthesis of the respective R-amine with high conversions by using either alanine or isopropylamine as amine donor. Asymmetric synthesis of (R)-2,2-dimethyl-1-phenylpropan-1-amine by amino group transfer to 2,2-dimethyl-1-phenylpropan-1-one catalyzed by ATAs. Isopropylamine or alanine serve as the amine donors. Analysis of specific activities of Rsp-ATA mutant variants towards rac-amine 2,2-dimethyl-1-phenylpropan-1-amine. Enzyme-ligand interaction analysis, overview | Ruegeria sp. TM1040 | ? | - |
- |
|
rac-2,2-dimethyl-1-phenylpropan-1-amine + pyruvate | - |
Ruegeria sp. TM1040 | 2,2-dimethyl-1-phenylpropan-1-one + L-alanine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
amine transaminase | - |
Ruegeria sp. TM1040 |
ATA | - |
Ruegeria sp. TM1040 |
Rsp-ATA | - |
Ruegeria sp. TM1040 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ruegeria sp. TM1040 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | assay at | Ruegeria sp. TM1040 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, dependent on | Ruegeria sp. TM1040 |
General Information | Comment | Organism |
---|---|---|
evolution | the ATA enzyme from Ruegeria sp. TM1040 (Rsp-ATA, PDB ID 3FCR) belongs to the ATAs of fold class I | Ruegeria sp. TM1040 |