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Literature summary for 2.6.1.B16 extracted from

  • Cerioli, L.; Planchestainer, M.; Cassidy, J.; Tessaro, D.; Paradisi, F.
    Characterization of a novel amine transaminase from Halomonas elongata (2015), J. Mol. Catal. B, 120, 141-150.
No PubMed abstract available
Search for more literature for 2.6.1.B16

General Stability

General Stability Organism
in 1 M KCl, 50% residual activity after 24 h Halomonas elongata
in 1 M NaCl, 40% residual activity after 24 h Halomonas elongata
the enzyme at concentrations greater than 5mM of cofactor pyridoxal 5'-phosphate loses the activity significantly faster than 0.1mM, confirming the destabilization effect Halomonas elongata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 pyruvate pH 8.0, 37°C Halomonas elongata
2.57
-
 (S)-1-phenylethylamine pH 8.0, 37°C Halomonas elongata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000, SDS-PAGE Halomonas elongata
109000
-
 gel filtration Halomonas elongata

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 20%, 27% residual activity after 22 h Halomonas elongata
Ethanol 20%, 25% residual activity after 22 h Halomonas elongata
isopropanol 20%, 16% residual activity after 22 h Halomonas elongata
Methanol 20%, 47% residual activity after 22 h Halomonas elongata
tert-Butanol 20%, 20% residual activity after 22 h Halomonas elongata

Organism

Organism UniProt Comment Textmining
Halomonas elongata E1V913
-
-
Halomonas elongata DSM 2581 E1V913
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.58
-
 substrate benzaldehyde, cosubstrate isopropylamine, pH 8.0, 37°C Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylpropionaldehyde + (S)-1-phenylethylamine 67% conversion of 2-phenylpropionaldehyde Halomonas elongata 2-phenylpropylamine + acetophenone
-
 ?
2-phenylpropionaldehyde + (S)-1-phenylethylamine 67% conversion of 2-phenylpropionaldehyde Halomonas elongata DSM 2581 2-phenylpropylamine + acetophenone
-
 ?
benzaldehyde + ortho-xylylenediamine 95% conversion of benzaldehyde Halomonas elongata benzylamine + ? conversion rate 90-95% ?
benzaldehyde + ortho-xylylenediamine 95% conversion of benzaldehyde Halomonas elongata DSM 2581 benzylamine + ? conversion rate 90-95% ?
cinnamaldehyde + (S)-1-phenylethylamine 56% conversion of cinnamaldehyde Halomonas elongata (2E)-3-phenylprop-2-en-1-amine + acetophenone
-
 ?
cinnamaldehyde + (S)-1-phenylethylamine 56% conversion of cinnamaldehyde Halomonas elongata DSM 2581 (2E)-3-phenylprop-2-en-1-amine + acetophenone
-
 ?
L-alanine + glyoxylate 41% conversion of alanine, 97% conversion of glyoxylate Halomonas elongata pyruvate + glycine
-
 ?
L-alanine + glyoxylate 41% conversion of alanine, 97% conversion of glyoxylate Halomonas elongata DSM 2581 pyruvate + glycine
-
 ?
propanal + (S)-1-phenylethylamine 55% conversion of propanal Halomonas elongata propylamine + acetophenone
-
 ?
pyruvate + (S)-1-phenylethylamine 97% conversion of (S)-1-phenylethylamine, less than 5% conversion of (R)-1-phenylethylamine Halomonas elongata L-alanine + acetophenone
-
 ?
pyruvate + benzylamine 64% conversion of isopropylamine Halomonas elongata L-alanine + benzaldehyde
-
 ?
pyruvate + glycine 97% conversion of pyruvate Halomonas elongata L-alanine + glyoxylate
-
 ?
pyruvate + glycine 97% conversion of pyruvate Halomonas elongata DSM 2581 L-alanine + glyoxylate
-
 ?
pyruvate + isopropylamine 41% conversion of isopropylamine Halomonas elongata L-alanine + acetone
-
 ?
pyruvate + L-serine 19% conversion of serine Halomonas elongata L-alanine + 3-hydroxy-2-oxopropanoate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Halomonas elongata

Synonyms

Synonyms Comment Organism
HELO 1904
-
 Halomonas elongata
HEWT
-
 Halomonas elongata
SpuC
-
 Halomonas elongata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Halomonas elongata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 24 h, stable up to 35°C Halomonas elongata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.094
-
 (S)-1-phenylethylamine pH 8.0, 37°C Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Halomonas elongata

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Halomonas elongata