BRENDA - Enzyme Database
show all sequences of 2.6.1.95

Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin

Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.; ChemBioChem 8, 283-288 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces fradiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48060
-
Neo-18, calculated from amino acid sequence
Streptomyces fradiae
48060
-
Neo-18, electrospray ionization mass spectrometry
Streptomyces fradiae
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces fradiae
-
-
-
Streptomyces fradiae NCIMB 8233
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni2+-NTA resin column chromatography
Streptomyces fradiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
6'-oxoglucosaminyl:L-glutamate aminotransferase
-
Streptomyces fradiae
Neo-18
-
Streptomyces fradiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces fradiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48060
-
Neo-18, calculated from amino acid sequence
Streptomyces fradiae
48060
-
Neo-18, electrospray ionization mass spectrometry
Streptomyces fradiae
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA resin column chromatography
Streptomyces fradiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
Other publictions for EC 2.6.1.95
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719676
Clausnitzer
The oxidoreductases LivQ and N ...
Streptomyces fradiae
J. Appl. Microbiol.
111
642-651
2011
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719294
Huang
Elaboration of neosamine rings ...
Streptomyces fradiae, Streptomyces fradiae NCIMB 8233
ChemBioChem
8
283-288
2007
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