Literature summary for 2.6.1.92 extracted from

Schoenhofen, I.C.; Lunin, V.V.; Julien, J.P.; Li, Y.; Ajamian, E.; Matte, A.; Cygler, M.; Brisson, J.R.; Aubry, A.; Logan, S.M.; Bhatia, S.; Wakarchuk, W.W.; Young, N.M.
Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori (2006), J. Biol. Chem., 281, 8907-8916.

Application

Application	Comment	Organism
medicine	the enzyme is involved biosynthesis of 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid (pseudaminic acid). Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Because motility is a key virulence factor for this and other important pathogens, the pseudaminic acid biosynthetic pathway offers potential for novel therapeutic targets	Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of the native protein, its complexes with pyridoxal 5'-phosphate alone and in combination with UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine. A crystal of apo-PseC is obtained in 2 days at 20°C from a hanging drop by mixing 0.0015 ml of protein in buffer with 0.0015 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.6, 30% polyethylene glycol 4000. The crystals belong to space group P2(1)2(1)2 with unit cell dimensions a = 87.7, b = 155.4, and c = 71.5 A and Z = 4	Helicobacter pylori

Crystallization (Comment)

Organism

crystal structure of the native protein, its complexes with pyridoxal 5'-phosphate alone and in combination with UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine. A crystal of apo-PseC is obtained in 2 days at 20°C from a hanging drop by mixing 0.0015 ml of protein in buffer with 0.0015 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.6, 30% polyethylene glycol 4000. The crystals belong to space group P2(1)2(1)2 with unit cell dimensions a = 87.7, b = 155.4, and c = 71.5 A and Z = 4

Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate	Helicobacter pylori	the enzyme is involved biosynthesis of pseudaminic acid. Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Motility is a key virulence factor for this and other important pathogens	UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate	-	?

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	O25130	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate	-	Helicobacter pylori	UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate	-	?
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate	the enzyme is involved biosynthesis of pseudaminic acid. Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Motility is a key virulence factor for this and other important pathogens	Helicobacter pylori	UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate	-	?

Subunits

Subunits	Comment	Organism
homodimer	PseC is a homodimer in solution (as shown by dynamic light scattering) as well as in the crystal structure, where the two monomers are related by 2fold crystallographic symmetry. The inter-subunit interactions are extensive, involving several elements of secondary structure, predominantly from the N-terminal domains of both monomers. Each monomer contributes to the active site	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
HP0366	-	Helicobacter pylori
PseC	-	Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Helicobacter pylori

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Helicobacter pylori

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	a pyridoxal 5'-phosphate protein	Helicobacter pylori

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved biosynthesis of 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid (pseudaminic acid). Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Motility is a key virulence factor for this and other important pathogens	Helicobacter pylori