BRENDA - Enzyme Database show
show all sequences of 2.6.1.9

Crystal structures of Mycobacterium tuberculosis HspAT and ArAT reveal structural basis of their distinct substrate specificities

Nasir, N.; Anant, A.; Vyas, R.; Biswal, B.K.; Sci. Rep. 6, 18880 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization
Organism
hydrophilic residues in the substrate binding pocket and N-terminal lid allow the entry and binding of its preferential substrate. The pyridoxal phosphate cofactor binds as an internal aldimine via a Schiff ’s base with epsilon-N of Lys232. The inhibitor 2-(N-morpholino)ethanesulfonic acid binds in the active site. The N-terminal lid is restructured upon binding of ligand, leading to a closed conformation of the enzyme necessary for the binding and probable catalysis of the substrate. The closing of the lid upon ligand binding causes Tyr25 to sweep into the active site region and interact with the ligand
Mycobacterium tuberculosis
Engineering
Amino acid exchange
Commentary
Organism
Y127F
about 20% of wild-type activity with L-histidinol phosphate, no significant loss of activity with phenylalanine
Mycobacterium tuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-(N-morpholino)ethanesulfonic acid
specific inhibitor
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.42
-
L-Histidinol phosphate
pH 8.4, 37°C
Mycobacterium tuberculosis
7.1
-
L-phenylalanine
pH 8.4, 37°C
Mycobacterium tuberculosis
9.8
-
L-tyrosine
pH 8.4, 37°C
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WML7
-
-
Mycobacterium tuberculosis H37Rv
P9WML7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-histidinol phosphate + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis H37Rv
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis
phenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis H37Rv
phenylpyruvate + L-glutamate
-
-
-
?
L-tyrosine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis
3-(4-hydroxyphenyl)-2-oxopropanoate + glutamate
-
-
-
?
L-tyrosine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis H37Rv
3-(4-hydroxyphenyl)-2-oxopropanoate + glutamate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
220
-
L-phenylalanine
pH 8.4, 37°C
Mycobacterium tuberculosis
426
-
L-Histidinol phosphate
pH 8.4, 37°C
Mycobacterium tuberculosis
3100
-
L-tyrosine
pH 8.4, 37°C
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hydrophilic residues in the substrate binding pocket and N-terminal lid allow the entry and binding of its preferential substrate. The pyridoxal phosphate cofactor binds as an internal aldimine via a Schiff ’s base with epsilon-N of Lys232. The inhibitor 2-(N-morpholino)ethanesulfonic acid binds in the active site. The N-terminal lid is restructured upon binding of ligand, leading to a closed conformation of the enzyme necessary for the binding and probable catalysis of the substrate. The closing of the lid upon ligand binding causes Tyr25 to sweep into the active site region and interact with the ligand
Mycobacterium tuberculosis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Y127F
about 20% of wild-type activity with L-histidinol phosphate, no significant loss of activity with phenylalanine
Mycobacterium tuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-(N-morpholino)ethanesulfonic acid
specific inhibitor
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.42
-
L-Histidinol phosphate
pH 8.4, 37°C
Mycobacterium tuberculosis
7.1
-
L-phenylalanine
pH 8.4, 37°C
Mycobacterium tuberculosis
9.8
-
L-tyrosine
pH 8.4, 37°C
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-histidinol phosphate + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis H37Rv
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis
phenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis H37Rv
phenylpyruvate + L-glutamate
-
-
-
?
L-tyrosine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis
3-(4-hydroxyphenyl)-2-oxopropanoate + glutamate
-
-
-
?
L-tyrosine + 2-oxoglutarate
-
739670
Mycobacterium tuberculosis H37Rv
3-(4-hydroxyphenyl)-2-oxopropanoate + glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
220
-
L-phenylalanine
pH 8.4, 37°C
Mycobacterium tuberculosis
426
-
L-Histidinol phosphate
pH 8.4, 37°C
Mycobacterium tuberculosis
3100
-
L-tyrosine
pH 8.4, 37°C
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
30
-
L-phenylalanine
pH 8.4, 37°C
Mycobacterium tuberculosis
320
-
L-tyrosine
pH 8.4, 37°C
Mycobacterium tuberculosis
1020
-
L-Histidinol phosphate
pH 8.4, 37°C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
30
-
L-phenylalanine
pH 8.4, 37°C
Mycobacterium tuberculosis
320
-
L-tyrosine
pH 8.4, 37°C
Mycobacterium tuberculosis
1020
-
L-Histidinol phosphate
pH 8.4, 37°C
Mycobacterium tuberculosis
Other publictions for EC 2.6.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739670
Nasir
Crystal structures of Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Sci. Rep.
6
18880
2016
-
-
1
1
1
-
1
3
-
-
-
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-
4
-
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6
-
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-
3
-
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1
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1
1
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1
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3
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6
-
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-
3
-
-
-
-
-
-
-
-
3
3
737370
Nasir
Sample preparation, crystalliz ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
69
445-448
2013
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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1
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1
-
1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721229
Nasir
Molecular cloning, overexpress ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. F
68
32-36
2012
-
-
1
1
-
-
-
-
-
-
-
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3
-
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1
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1
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1
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1
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-
684151
Marienhagen
Insights into the structural b ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. D
64
675-685
2008
-
-
-
1
4
-
-
15
-
-
-
-
-
4
-
-
-
-
-
-
8
-
9
-
-
-
-
15
-
-
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-
-
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-
-
-
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1
4
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
8
-
9
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
676602
Mo
The hpa1 mutant of Arabidopsis ...
Arabidopsis thaliana
Plant Physiol.
141
1425-1435
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
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-
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-
-
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-
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1
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1
-
-
1
-
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-
-
-
-
-
-
-
-
-
659343
Fernandez
Structural studies of the cata ...
Thermotoga maritima
J. Biol. Chem.
279
21478-21488
2004
-
-
1
1
-
-
-
4
-
-
-
2
-
2
-
-
1
1
-
-
-
-
7
-
1
-
-
4
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
4
-
-
-
2
-
-
-
1
-
-
-
-
7
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
637153
Mizuguchi
Characterization of histidinol ...
Escherichia coli
Biochim. Biophys. Acta
1647
321-324
2003
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
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1
-
-
-
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1
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4
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1
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-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
637150
El Malki
Molecular characterization and ...
Nicotiana tabacum
Plant Mol. Biol.
45
191-199
2001
-
-
1
-
-
-
-
-
1
-
2
-
-
2
-
-
-
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-
-
1
-
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-
-
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1
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1
-
2
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637152
Haruyama
Structures of Escherichia coli ...
Escherichia coli
Biochemistry
40
4633-4644
2001
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
1
-
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-
1
-
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1
-
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-
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637154
Sivaraman
Crystal structure of histidino ...
Escherichia coli
J. Mol. Biol.
311
761-776
2001
-
-
1
1
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
1
1
-
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-
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1
-
1
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1
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-
1
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-
-
1
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
637151
Gu
Imidazole acetol phosphate ami ...
Zymomonas mobilis
J. Bacteriol.
177
1576-1584
1995
-
-
1
-
-
-
-
3
-
-
2
-
-
1
-
-
1
1
-
-
4
3
9
1
-
-
2
-
1
-
-
1
-
-
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-
-
1
1
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
4
3
9
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
637144
Hsu
L-Histidinol phosphate aminotr ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochimie
71
477-489
1989
-
-
-
-
-
-
-
-
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2
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1
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1
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1
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1
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1
-
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-
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-
-
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-
-
-
-
636659
Weigent
Purification and properties of ...
Bacillus subtilis
J. Biol. Chem.
251
6974-6980
1976
-
-
-
-
-
-
1
4
-
-
1
-
-
2
-
-
1
1
-
-
1
1
3
-
-
-
1
-
1
-
1
1
-
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-
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-
-
1
-
-
-
-
1
-
4
-
-
1
-
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-
1
-
-
1
1
3
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
637147
Henderson
Omega-aminoalkylagaroses in th ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
13
4335-4338
1974
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
637146
Henderson
Crystalline L-histidinol phosp ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
1906-1911
1973
-
-
-
1
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
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1
-
1
1
1
-
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-
-
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-
1
-
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1
1
-
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-
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-
2
-
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-
1
-
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1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637149
Roberts
-
Imidazolylacetolphosphate amin ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
77408-7753
1973
-
-
-
-
-
-
-
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1
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1
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1
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2
-
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1
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1
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1
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1
-
-
-
-
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-
-
2
-
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-
-
-
-
-
-
-
-
637143
Martin
Imidazolylacetolphosphate:L-gl ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1168-1174
1967
-
-
-
-
-
-
-
-
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1
1
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1
-
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1
-
-
-
1
-
2
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
637145
Martin
Imidazolylacetolphosphate:L-gl ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1175-1181
1967
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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1
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637142
Ames
Biosynthesis of histidine: imi ...
Neurospora sp.
J. Biol. Chem.
220
113-128
1956
1
-
-
-
-
-
5
2
-
-
-
-
-
1
-
-
1
-
-
-
1
1
8
-
1
-
-
-
3
1
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
2
-
-
-
-
-
-
-
1
-
-
1
1
8
-
1
-
-
-
3
1
-
-
-
-
-
-
-
-