BRENDA - Enzyme Database show
show all sequences of 2.6.1.9

Purification and properties of two aromatic aminotransferases in Bacillus subtilis

Weigent, D.A.; Nester, E.W.; J. Biol. Chem. 251, 6974-6980 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
L-glutamate
competitive to tyrosine
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
L-Histidinol phosphate
-
Bacillus subtilis
0.71
-
L-tyrosine
-
Bacillus subtilis
2.5
-
L-phenylalanine
-
Bacillus subtilis
7.1
-
2-oxoglutarate
-
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Purification (Commentary)
Commentary
Organism
220fold
Bacillus subtilis
Reaction
Reaction
Commentary
Organism
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
ping-pong bi bi mechanism
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
325
-
-
Bacillus subtilis
Storage Stability
Storage Stability
Organism
0°C, 1 month, 30% loss of activity
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-histidinol phosphate + 2-oxoglutarate
-
636659
Bacillus subtilis
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
636659
Bacillus subtilis
phenylpyruvate + L-glutamate
-
-
-
?
L-tryptophan + 2-oxoglutarate
-
636659
Bacillus subtilis
3-indole-2-oxopropanoate + L-glutamate
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
4 min, more than 90% loss of activity, 10 min, in presence of pyridoxal 5'-phosphate, 75% loss of activity
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
-
Bacillus subtilis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8.5
stable overnight
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
0.0022 mM; a pyridoxal phosphate protein
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
0.0022 mM; a pyridoxal phosphate protein
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-glutamate
competitive to tyrosine
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
L-Histidinol phosphate
-
Bacillus subtilis
0.71
-
L-tyrosine
-
Bacillus subtilis
2.5
-
L-phenylalanine
-
Bacillus subtilis
7.1
-
2-oxoglutarate
-
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
220fold
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
325
-
-
Bacillus subtilis
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 1 month, 30% loss of activity
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-histidinol phosphate + 2-oxoglutarate
-
636659
Bacillus subtilis
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
636659
Bacillus subtilis
phenylpyruvate + L-glutamate
-
-
-
?
L-tryptophan + 2-oxoglutarate
-
636659
Bacillus subtilis
3-indole-2-oxopropanoate + L-glutamate
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
4 min, more than 90% loss of activity, 10 min, in presence of pyridoxal 5'-phosphate, 75% loss of activity
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
-
Bacillus subtilis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8.5
stable overnight
Bacillus subtilis
Other publictions for EC 2.6.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739670
Nasir
Crystal structures of Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Sci. Rep.
6
18880
2016
-
-
1
1
1
-
1
3
-
-
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-
-
4
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6
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3
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1
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1
1
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1
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3
-
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-
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-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
737370
Nasir
Sample preparation, crystalliz ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
69
445-448
2013
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
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1
-
1
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-
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1
-
-
-
-
-
-
-
-
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-
-
-
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-
721229
Nasir
Molecular cloning, overexpress ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. F
68
32-36
2012
-
-
1
1
-
-
-
-
-
-
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-
-
3
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1
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1
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1
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1
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-
-
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-
-
-
-
-
684151
Marienhagen
Insights into the structural b ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. D
64
675-685
2008
-
-
-
1
4
-
-
15
-
-
-
-
-
4
-
-
-
-
-
-
8
-
9
-
-
-
-
15
-
-
-
-
-
-
-
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-
-
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1
4
-
-
-
-
15
-
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-
-
-
-
-
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-
-
8
-
9
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
676602
Mo
The hpa1 mutant of Arabidopsis ...
Arabidopsis thaliana
Plant Physiol.
141
1425-1435
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
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1
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1
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1
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1
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1
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-
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-
-
-
659343
Fernandez
Structural studies of the cata ...
Thermotoga maritima
J. Biol. Chem.
279
21478-21488
2004
-
-
1
1
-
-
-
4
-
-
-
2
-
2
-
-
1
1
-
-
-
-
7
-
1
-
-
4
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
4
-
-
-
2
-
-
-
1
-
-
-
-
7
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
637153
Mizuguchi
Characterization of histidinol ...
Escherichia coli
Biochim. Biophys. Acta
1647
321-324
2003
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
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1
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1
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4
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1
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-
637150
El Malki
Molecular characterization and ...
Nicotiana tabacum
Plant Mol. Biol.
45
191-199
2001
-
-
1
-
-
-
-
-
1
-
2
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2
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1
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1
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1
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2
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1
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637152
Haruyama
Structures of Escherichia coli ...
Escherichia coli
Biochemistry
40
4633-4644
2001
-
-
-
1
-
-
-
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-
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1
-
3
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2
1
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1
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1
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2
1
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-
637154
Sivaraman
Crystal structure of histidino ...
Escherichia coli
J. Mol. Biol.
311
761-776
2001
-
-
1
1
-
-
-
-
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-
1
-
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6
-
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1
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1
1
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1
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1
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1
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1
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1
1
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-
637151
Gu
Imidazole acetol phosphate ami ...
Zymomonas mobilis
J. Bacteriol.
177
1576-1584
1995
-
-
1
-
-
-
-
3
-
-
2
-
-
1
-
-
1
1
-
-
4
3
9
1
-
-
2
-
1
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1
-
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-
1
1
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-
-
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3
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2
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1
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4
3
9
1
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2
-
1
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637144
Hsu
L-Histidinol phosphate aminotr ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochimie
71
477-489
1989
-
-
-
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2
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1
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1
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636659
Weigent
Purification and properties of ...
Bacillus subtilis
J. Biol. Chem.
251
6974-6980
1976
-
-
-
-
-
-
1
4
-
-
1
-
-
2
-
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1
1
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1
1
3
-
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1
-
1
-
1
1
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-
1
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1
-
4
-
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1
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1
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1
1
3
-
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1
-
1
-
1
-
-
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-
-
-
-
637147
Henderson
Omega-aminoalkylagaroses in th ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
13
4335-4338
1974
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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637146
Henderson
Crystalline L-histidinol phosp ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
1906-1911
1973
-
-
-
1
-
-
-
-
-
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2
-
-
2
-
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1
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1
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1
1
1
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1
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1
1
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2
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1
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1
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1
1
1
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637149
Roberts
-
Imidazolylacetolphosphate amin ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
77408-7753
1973
-
-
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1
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1
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1
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2
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637143
Martin
Imidazolylacetolphosphate:L-gl ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1168-1174
1967
-
-
-
-
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1
1
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1
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1
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1
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1
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1
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1
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2
1
1
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1
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637145
Martin
Imidazolylacetolphosphate:L-gl ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1175-1181
1967
-
-
-
-
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1
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1
1
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1
1
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637142
Ames
Biosynthesis of histidine: imi ...
Neurospora sp.
J. Biol. Chem.
220
113-128
1956
1
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5
2
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1
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1
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1
1
8
-
1
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3
1
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1
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1
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1
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5
-
2
-
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-
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1
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1
1
8
-
1
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3
1
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