KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
2-oxo-4-methylthiobutanoate | recombinant enzyme, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.93 | - |
L-methionine | recombinant enzyme, pH and temperature not specified in the publication | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LE06 | ecotype Col-0 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
phloem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methylthiobutanoate + an L-amino acid | recombinant enzyme shows high activity with 2-oxo-4-methylthiobutanoate (100% activity at 0.1 and 2 mM) | Arabidopsis thaliana | L-methionine + a 2-oxo acid | - |
r | |
4-methyl-2-oxopentanoate + ? | 41% activity at 2 mM compared to 2-oxo-4-methylthiobutanoate | Arabidopsis thaliana | L-methionine + a 2-oxo acid | - |
r | |
5-methylthio-2-oxopentanoate + ? | 48% activity at 0.1 mM and 53% activity at 2 mM compared to 2-oxo-4-methylthiobutanoate | Arabidopsis thaliana | L-methionine + a 2-oxo acid | - |
r | |
L-homomethionine + a 2-oxo acid | 37% activity at 1 mM and 65% activity at 5 mM compared to L-methionine | Arabidopsis thaliana | ? | - |
r | |
L-leucine + a 2-oxo acid | 29% activity at 1 mM and 62% activity at 5 mM compared to 2-oxo-4-methylthiobutanoate | Arabidopsis thaliana | 2-oxo-4-methylthiobutanoate + an L-amino acid | - |
r | |
L-methionine + a 2-oxo acid | recombinant enzyme shows high efficiency with L-methionine (100% activity at 1 and 5 mM) | Arabidopsis thaliana | 2-oxo-4-methylthiobutanoate + an L-amino acid | - |
r | |
additional information | the enzyme does not use L-isoleucine (at 1 mM), L-valine (at 1 and 5 mM), 4-methyl-2-oxopentanoate (at 0.1 mM), 6-methylthio-2-oxohexanoate, 3-methyl-2-oxopentanoate, and 3-methyl-2-oxobutyrate as substrates (at 0.1 mM each) | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCAT4 | - |
Arabidopsis thaliana |
branched-chain aminotransferase4 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | branched-chain aminotransferase4 transcription is induced by wounding. Branched-chain aminotransferase4 mRNA accumulation is light dependent | up |
General Information | Comment | Organism |
---|---|---|
metabolism | branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis | Arabidopsis thaliana |