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Literature summary for 2.6.1.88 extracted from

  • Schuster, J.; Knill, T.; Reichelt, M.; Gershenzon, J.; Binder, S.
    Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis (2006), Plant Cell, 18, 2664-2679.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
2-oxo-4-methylthiobutanoate recombinant enzyme, pH and temperature not specified in the publication Arabidopsis thaliana
0.93
-
L-methionine recombinant enzyme, pH and temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LE06 ecotype Col-0
-

Source Tissue

Source Tissue Comment Organism Textmining
phloem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-methylthiobutanoate + an L-amino acid recombinant enzyme shows high activity with 2-oxo-4-methylthiobutanoate (100% activity at 0.1 and 2 mM) Arabidopsis thaliana L-methionine + a 2-oxo acid
-
r
4-methyl-2-oxopentanoate + ? 41% activity at 2 mM compared to 2-oxo-4-methylthiobutanoate Arabidopsis thaliana L-methionine + a 2-oxo acid
-
r
5-methylthio-2-oxopentanoate + ? 48% activity at 0.1 mM and 53% activity at 2 mM compared to 2-oxo-4-methylthiobutanoate Arabidopsis thaliana L-methionine + a 2-oxo acid
-
r
L-homomethionine + a 2-oxo acid 37% activity at 1 mM and 65% activity at 5 mM compared to L-methionine Arabidopsis thaliana ?
-
r
L-leucine + a 2-oxo acid 29% activity at 1 mM and 62% activity at 5 mM compared to 2-oxo-4-methylthiobutanoate Arabidopsis thaliana 2-oxo-4-methylthiobutanoate + an L-amino acid
-
r
L-methionine + a 2-oxo acid recombinant enzyme shows high efficiency with L-methionine (100% activity at 1 and 5 mM) Arabidopsis thaliana 2-oxo-4-methylthiobutanoate + an L-amino acid
-
r
additional information the enzyme does not use L-isoleucine (at 1 mM), L-valine (at 1 and 5 mM), 4-methyl-2-oxopentanoate (at 0.1 mM), 6-methylthio-2-oxohexanoate, 3-methyl-2-oxopentanoate, and 3-methyl-2-oxobutyrate as substrates (at 0.1 mM each) Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
BCAT4
-
Arabidopsis thaliana
branched-chain aminotransferase4
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana branched-chain aminotransferase4 transcription is induced by wounding. Branched-chain aminotransferase4 mRNA accumulation is light dependent up

General Information

General Information Comment Organism
metabolism branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis Arabidopsis thaliana