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Literature summary for 2.6.1.86 extracted from
- Expression and characterization of PhzE from P. aeruginosa PAO1: aminodeoxyisochorismate synthase involved in pyocyanin and phenazine-1-carboxylate production (2013), Biochim. Biophys. Acta, 1834, 240-246.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
chorismate | L-glutamine as ammonia source, in the presence of Mg2+, pH 7.0, 25°C | Pseudomonas aeruginosa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | lower activity with Mn2+, Co2+, no detectable activity with Zn2+, Fe2+, Ca2+ | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71251 | - |
x * 71251, His-tagged PhzE, MALDI-TOF | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | G3XCK6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + chorismate | - |
Pseudomonas aeruginosa | (S)-2-amino-2-deoxyisochorismate + glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71251, His-tagged PhzE, MALDI-TOF | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminodeoxyisochorismate synthase | - |
Pseudomonas aeruginosa |
| PhzE | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 45 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
chorismate | ammonium sulfate as ammonia source, pH 7.0, 25°C | Pseudomonas aeruginosa | |
| 2.2 | - |
chorismate | L-glutamine as ammonia source, pH 7.0, 25°C | Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
optimal activity at low pH values between pH 6.5 and pH 8.5 | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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