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Literature summary for 2.6.1.85 extracted from
- Cloning, expression, and characterization of para-aminobenzoic acid (PABA) synthase from Agaricus bisporus 02, a thermotolerant mushroom strain (2015), J. Microbiol. Biotechnol., 25, 66-73.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Agaricus bisporus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.117 | - |
chorismate | pH 8.0, 25°C | Agaricus bisporus |  |
| 2.4 | - |
L-glutamine | pH 8.0, 25°C | Agaricus bisporus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, in decreasing activity: Mg2+, Mn2+, Co2+ | Agaricus bisporus | |
| Mg2+ | divalent cation required, in decreasing activity: Mg2+, Mn2+, Co2+. Highest activity at 5 mM Mg2+ | Agaricus bisporus | |
| Mn2+ | divalent cation required, in decreasing activity: Mg2+, Mn2+, Co2+ | Agaricus bisporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80600 | - |
- |
Agaricus bisporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | A0A060IGN1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-asparagine | 11% of the activity with L-glutamine | Agaricus bisporus | 4-amino-4-deoxychorismate + L-aspartate | - |
? | |
| chorismate + L-glutamine | - |
Agaricus bisporus | 4-amino-4-deoxychorismate + L-glutamate | - |
? | |
| chorismate + NH3 | 30% of the activity with L-glutamine | Agaricus bisporus | 4-amino-4-deoxychorismate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80600, calculated | Agaricus bisporus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PabAB | - |
Agaricus bisporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Agaricus bisporus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
L-glutamine | pH 8.0, 25°C | Agaricus bisporus | |
| 0.017 | - |
chorismate | pH 8.0, 25°C | Agaricus bisporus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Agaricus bisporus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
L-glutamine | pH 8.0, 25°C | Agaricus bisporus | |
| 143 | - |
chorismate | pH 8.0, 25°C | Agaricus bisporus | |
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