Cloned (Comment) | Organism |
---|---|
gene aruH, overexpression of soluble His-tagged enzyme in Escherichia coli | Pseudomonas aeruginosa |
overexpressed in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
borate/NaOH buffer | strong inhibition at pH 9.0 | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pseudomonas aeruginosa | |
additional information | - |
additional information | catalytic efficiency kcat/KM 24.1 mM/s for pyruvate and 2.8 mM/s for arginine | Pseudomonas aeruginosa | |
1.6 | - |
pyruvate | pH 9.0, 37°C | Pseudomonas aeruginosa | |
1.6 | - |
pyruvate | pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase | Pseudomonas aeruginosa | |
2.8 | - |
L-arginine | pH 9.0, 37°C | Pseudomonas aeruginosa | |
13.9 | - |
L-arginine | pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
43000 | - |
2 * 43000, SDS-PAGE, His-tagged | Pseudomonas aeruginosa |
79300 | - |
recombinant enzyme, gel filtration | Pseudomonas aeruginosa |
79300 | - |
native enzyme, gel filtration, apparent mass, indicating that His-tagged AruH is a homodimer | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + pyruvate | Pseudomonas aeruginosa | AruH catalyzes the first step of the arginine transaminase pathway, representing one of the multiple pathways for L-arginine catabolism | 5-guanidino-2-oxopentanoate + L-alanine | - |
? | |
L-arginine + pyruvate | Pseudomonas aeruginosa | AruH catalyzes the first step in the arginine transaminase pathway, converting the substrates L-arginine and pyruvate into 2-ketoarginine and L-alanine, AruH may have a broader physiological function in amino acid catabolism | 5-guanidino-2-oxopentanoate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | - |
gene aruH | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, to homogeneity, using HisTrap HP kit, anion exchange chromatography Mono Q | Pseudomonas aeruginosa |
recombinant His-tagged AruH from Escherichia coli to homogeneity by affinity and anion exchange chromatography | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L-alanine | ping-pong kinetic mechanism | Pseudomonas aeruginosa | |
L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L-alanine | AruH catalyzes the first step of the arginine transaminase pathway, representing one of the multiple pathways for L-arginine catabolism | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
a coupled reaction assay with NAD+ and L-alanine dehydrogenase | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + pyruvate | - |
Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + L-alanine | product identification by mass spectrometry | ? | |
L-arginine + pyruvate | AruH catalyzes the first step of the arginine transaminase pathway, representing one of the multiple pathways for L-arginine catabolism | Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + L-alanine | - |
? | |
L-arginine + pyruvate | broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine | Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + L-alanine | detected by HPLC and mass spectrometry | ? | |
L-arginine + pyruvate | AruH catalyzes the first step in the arginine transaminase pathway, converting the substrates L-arginine and pyruvate into 2-ketoarginine and L-alanine, AruH may have a broader physiological function in amino acid catabolism | Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + L-alanine | - |
? | |
L-glutamine + pyruvate | broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine | Pseudomonas aeruginosa | 2-oxoglutaramate + L-alanine | - |
? | |
L-glutamine + pyruvate | - |
Pseudomonas aeruginosa | ? | - |
? | |
L-leucine + pyruvate | - |
Pseudomonas aeruginosa | 4-methyl-2-oxopentanoate + L-alanine | - |
? | |
L-leucine + pyruvate | broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine | Pseudomonas aeruginosa | 4-methyl-2-oxopentanoate + L-alanine | - |
? | |
L-lysine + pyruvate | broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine | Pseudomonas aeruginosa | DELTA1-piperidine-2-carboxylate + L-alanine | mass spectrometry analysis, implies that AruH may have broader physiological functions in amino acid catabolism | ? | |
L-lysine + pyruvate | - |
Pseudomonas aeruginosa | DELTA1-piperideine-2-carboxylate + L-alanine | product identification by mass spectrometry | ? | |
L-methionine + pyruvate | broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine | Pseudomonas aeruginosa | 4-methylthio-2-oxobutanoate + L-alanine | - |
? | |
L-methionine + pyruvate | - |
Pseudomonas aeruginosa | 4-methylthio-2-oxo-butanoate + L-alanine | - |
? | |
L-ornithine + pyruvate | broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine | Pseudomonas aeruginosa | 5-amino-2-oxopentanoate + L-alanine | - |
? | |
L-ornithine + pyruvate | - |
Pseudomonas aeruginosa | ? | - |
? | |
additional information | broad substrate specificity in an descending order of preference: Arg, Lys, Met, Leu, Orn, Gln | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
homodimer | 2 * 43000, SDS-PAGE, His-tagged | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
arginine:pyruvate transaminase | - |
Pseudomonas aeruginosa |
AruH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Pseudomonas aeruginosa |
42 | - |
sharp inactivation above | Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 42 | the enzyme is prone to sharp thermal inactivation above this temperature | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | catalytic efficiency kcat/KM 24.1 mM/s for pyruvate and 2.8 mM/s for arginine | Pseudomonas aeruginosa | |
38.6 | - |
L-arginine | pH 9.0, 37°C | Pseudomonas aeruginosa | |
38.6 | - |
pyruvate | calculated, pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase | Pseudomonas aeruginosa | |
38.6 | - |
L-arginine | calculated, pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas aeruginosa |
9 | - |
in borate/NaOH buffer | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | activity range | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Pseudomonas aeruginosa |