Cloned (Comment) | Organism |
---|---|
S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in complementation experiments | Chlamydomonas reinhardtii |
Crystallization (Comment) | Organism |
---|---|
PEG 3350, sitting drop method | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
tetrahydrodipicolinate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
0.3 | - |
LL-diaminopimelate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
0.9 | - |
L-glutamate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
2.2 | - |
2-oxoglutarate | pH 7.6, 30°C | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE, analytical ultracentrifugation | Chlamydomonas reinhardtii |
100200 | - |
analytical ultracentrifugation | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8IW39 | - |
- |
Chlamydomonas reinhardtii CC-1690 | A8IW39 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography, gel filtration | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.68 | - |
substrates glutamate and tetrahydrodipicolinate, pH 7.6, 30°C | Chlamydomonas reinhardtii |
11.6 | - |
substrates LL-diaminopimelate and 2-oxoglutarate, pH 7.6, 30°C | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + tetrahydrodipicolinate + H2O | no activity observed with pyruvate, prephenate, oxaloacetate and oxovalerate | Chlamydomonas reinhardtii | LL-diaminopimelate + 2-oxoglutarate | - |
r | |
L-glutamate + tetrahydrodipicolinate + H2O | no activity observed with pyruvate, prephenate, oxaloacetate and oxovalerate | Chlamydomonas reinhardtii CC-1690 | LL-diaminopimelate + 2-oxoglutarate | - |
r | |
LL-diaminopimelate + 2-oxoglutarate | no activity observed with meso-diaminopimelate, L-lysine and L-ornithine | Chlamydomonas reinhardtii | L-glutamate + tetrahydrodipicolinate + H2O | - |
r | |
LL-diaminopimelate + 2-oxoglutarate | no activity observed with meso-diaminopimelate, L-lysine and L-ornithine | Chlamydomonas reinhardtii CC-1690 | L-glutamate + tetrahydrodipicolinate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE, analytical ultracentrifugation | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
L,L-diaminopimelate aminotransferase | - |
Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
tetrahydrodipicolinate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
19 | - |
LL-diaminopimelate | pH 7.6, 30°C | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Chlamydomonas reinhardtii |