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Literature summary for 2.6.1.83 extracted from

  • Watanabe, N.; Cherney, M.M.; van Belkum, M.J.; Marcus, S.L.; Flegel, M.D.; Clay, M.D.; Deyholos, M.K.; Vederas, J.C.; James, M.N.
    Crystal structure of LL-diaminopimelate aminotransferase from Arabidopsis thaliana: a recently discovered enzyme in the biosynthesis of L-lysine by plants and chlamydia (2007), J. Mol. Biol., 371, 685-702.
    View publication on PubMed
Search for more literature for 2.6.1.83

Cloned(Commentary)

Cloned (Comment) Organism
cDNA sequence of LL-DAP-AT from Arabidopsis thaliana is optimized for expression in bacteria and cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification Arabidopsis thaliana
cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Arabidopsis thaliana
hanging drop vapour diffusion method at room temperature, 1.95 A resolution. The structure of AtDAP-AT is determined using the multiple-wavelength anomalous dispersion method with a seleno-methionine derivative Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana enzyme in the biosynthesis of L-lysine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93ZN9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 Arabidopsis thaliana LL-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 ?
additional information enzyme in the biosynthesis of L-lysine Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtDAP-AT
-
 Arabidopsis thaliana
LL-DAP-AT
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on. Each subunit of the dimer has pyridoxal-5'-phosphate in the active site Arabidopsis thaliana