Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli B834 (DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, PEG 3350, pH 7.5 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50700 | - |
2 * 50700, gel filtration | Escherichia coli |
100000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P42588 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography, gel filtration | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50700, gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
putrescine:2-oxoglutarate aminotransferase | - |
Escherichia coli |
YgjG | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |