BRENDA - Enzyme Database
show all sequences of 2.6.1.82

Molecular cloning and characterization of Escherichia coli K12 ygjG gene

Samsonova, N.N.; Smirnov, S.V.; Altman, I.B.; Ptitsyn, L.R.; BMC Microbiol. 3, 2 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli TG1 and BL21 (DE3)
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
9.2
-
putrescine
-
Escherichia coli
19
-
2-oxoglutaric acid
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
SDS-PAGE, 49600 Da for ORF2 coding protein and 2100 Da for His6-tag leader peptide
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P42588
K12; MG1655 (K-12 lambda-F-)
-
Purification (Commentary)
Commentary
Organism
95% purified by immobilized-metal affinity chromatography
Escherichia coli
Reaction
Reaction
Commentary
Organism
4-aminobutanal = 1-pyrroline + H2O
(1b)
Escherichia coli
putrescine + 2-oxoglutarate = 4-aminobutanal + L-glutamate
(1a)
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.047
-
highest activity in cell extracts grown under nitrogen limitation
Escherichia coli
2.16
-
essential level of glutamate formation activity in crude cells of BL21(DE3) harboring pET-Ht-ORF2
Escherichia coli
11.68
-
purified enzyme with yield of 81%
Escherichia coli
Storage Stability
Storage Stability
Organism
-70°C, phosphate buffer, pH 7.4, 15% glycerol, until required
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
agmatine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
alpha-ketobutyric acid + putrescine
38% activity
661399
Escherichia coli
L-aspartate + 4-aminobutanal
-
-
-
?
cadaverine + 2-oxoglutarate
97% activity
661399
Escherichia coli
L-glutamate + 5-aminopentanal
-
-
-
?
L-ornithine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
putrescine + 2-oxoglutarate
putrescine best amino group donor for the enzyme, 100% activity
661399
Escherichia coli
L-glutamate + 4-aminobutanal
-
-
-
?
pyruvate + putrescine
12% activity
661399
Escherichia coli
L-alanine + 4-aminobutanal
-
-
-
?
spermidine + 2-oxoglutarate
32% activity
661399
Escherichia coli
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Escherichia coli
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
-
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
in Tris-HCl buffer
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli TG1 and BL21 (DE3)
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
9.2
-
putrescine
-
Escherichia coli
19
-
2-oxoglutaric acid
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
SDS-PAGE, 49600 Da for ORF2 coding protein and 2100 Da for His6-tag leader peptide
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
95% purified by immobilized-metal affinity chromatography
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.047
-
highest activity in cell extracts grown under nitrogen limitation
Escherichia coli
2.16
-
essential level of glutamate formation activity in crude cells of BL21(DE3) harboring pET-Ht-ORF2
Escherichia coli
11.68
-
purified enzyme with yield of 81%
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, phosphate buffer, pH 7.4, 15% glycerol, until required
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
agmatine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
alpha-ketobutyric acid + putrescine
38% activity
661399
Escherichia coli
L-aspartate + 4-aminobutanal
-
-
-
?
cadaverine + 2-oxoglutarate
97% activity
661399
Escherichia coli
L-glutamate + 5-aminopentanal
-
-
-
?
L-ornithine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
putrescine + 2-oxoglutarate
putrescine best amino group donor for the enzyme, 100% activity
661399
Escherichia coli
L-glutamate + 4-aminobutanal
-
-
-
?
pyruvate + putrescine
12% activity
661399
Escherichia coli
L-alanine + 4-aminobutanal
-
-
-
?
spermidine + 2-oxoglutarate
32% activity
661399
Escherichia coli
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
-
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
in Tris-HCl buffer
Escherichia coli
Other publictions for EC 2.6.1.82
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737428
Jorge
A new metabolic route for the ...
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1
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738065
Slabu
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Putrescine transaminases for t ...
Bacillus megaterium, Bacillus megaterium WSH-002, Bacillus mycoides, Escherichia coli
ChemCatChem
8
1038-1042
2016
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3
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4
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4
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3
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4
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737567
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Two mechanisms for putrescine- ...
Escherichia coli
Arch. Microbiol.
196
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1
1
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-
739501
Cha
Structure of putrescine aminot ...
Escherichia coli
PLoS ONE
9
e113212
2014
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1
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1
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1
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723860
Yeo
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Escherichia coli
Acta Crystallogr. Sect. F
68
1070-1072
2012
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1
1
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2
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2
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1
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1
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1
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1
1
1
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-
-
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2
-
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1
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1
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-
703447
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Modification of PATase by L/F- ...
Escherichia coli
EMBO J.
28
1732-1744
2009
-
-
-
-
-
-
-
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1
-
-
1
-
4
-
1
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-
-
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1
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1
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1
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1
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1
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661760
Samsonova
Identification of Escherichia ...
Escherichia coli
FEBS Lett.
579
4107-4112
2005
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3
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1
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1
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1
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1
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661399
Samsonova
Molecular cloning and characte ...
Escherichia coli
BMC Microbiol.
3
2
2003
-
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1
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-
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2
-
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1
-
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1
-
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1
2
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3
1
7
-
1
1
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1
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1
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2
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1
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1
-
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3
1
7
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1
1
-
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1
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-
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-
288070
Prieto-Santos
A pathway for putrescine catab ...
Escherichia coli
Biochim. Biophys. Acta
880
242-244
1986
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1
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2
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6
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1
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1
1
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1
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6
-
1
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1
1
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