Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxyphenyllactic acid | metabolite of rosmarinic acid | Anchusa officinalis | |
additional information | enzyme shows substrate inhibition, but no feedback inhibition by L-phenylalanine and L-tyrosine | Anchusa officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-aspartate | pH 9.0, 40°C | Anchusa officinalis | |
0.08 | - |
prephenate | pH 9.0, 40°C | Anchusa officinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE | Anchusa officinalis |
57000 | - |
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE | Anchusa officinalis |
220000 | - |
gel filtration | Anchusa officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + L-aspartate | Anchusa officinalis | transamination | L-arogenate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anchusa officinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 41fold to homogeneity by anion exchange chromatography, chromatofocusing using the major active fraction with an isoelectric point of 5.4, and gel filtration | Anchusa officinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | rosmarinic acid-producing cells | Anchusa officinalis | - |
additional information | activity during cell cycle | Anchusa officinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
purified enzyme | Anchusa officinalis |
Storage Stability | Organism |
---|---|
-70°C, purified enzyme, 50 mM Tris-HCl, pH 8.2, 5 mM 2-mercaptoethanol, 150 mM sucrose, 1 mM EDTA, stable for at least 5 months | Anchusa officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-aspartate | - |
Anchusa officinalis | L-glutamate + oxaloacetate | - |
? | |
4-hydroxyphenylpyruvate + L-aspartate | - |
Anchusa officinalis | L-tyrosine + oxaloacetate | - |
? | |
glyoxylate + L-aspartate | - |
Anchusa officinalis | glycine + oxaloacetate | - |
? | |
indolepyruvate + L-aspartate | - |
Anchusa officinalis | L-tryptophan + oxaloacetate | - |
? | |
additional information | substrate specificity, highest activity with 2-oxoglutarate and L-aspartate as substrates, low activity with indolepyruvate and glyoxylate | Anchusa officinalis | ? | - |
? | |
phenylpyruvate + L-aspartate | - |
Anchusa officinalis | L-phenylalanine + oxaloacetate | - |
? | |
prephenate + L-aspartate | transamination | Anchusa officinalis | L-arogenate + oxaloacetate | - |
? | |
prephenate + L-glutamate | transamination, L-glutamate is about 3fold less effective than L-aspartate | Anchusa officinalis | L-arogenate + 2-oxoglutarate | - |
? | |
pyruvate + L-aspartate | - |
Anchusa officinalis | L-alanine + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE | Anchusa officinalis |
Synonyms | Comment | Organism |
---|---|---|
Pat | - |
Anchusa officinalis |
prephenate aminotransferase | - |
Anchusa officinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Anchusa officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Anchusa officinalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Anchusa officinalis | minor fraction, chromatofocussing | - |
5 |
Anchusa officinalis | major fraction, chromatofocussing | - |
5.4 |