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Literature summary for 2.6.1.76 extracted from

  • Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.
    Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata (1999), J. Bacteriol., 181, 91-99.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
 L-aspartate 4-semialdehyde pH 8.5, 25°C Halomonas elongata
9.1
-
 L-glutamate pH 8.5, 25°C Halomonas elongata

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Halomonas elongata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 6 * 44000, SDS-PAGE Halomonas elongata
260000
-
 gel filtration Halomonas elongata

Organism

Organism UniProt Comment Textmining
Halomonas elongata O52250
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halomonas elongata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 pH 8.5, 25°C Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate about 60% of the activity with L-2,4-diaminobutanoate Halomonas elongata ? + L-glutamate
-
 r
L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Halomonas elongata L-aspartate 4-semialdehyde + L-glutamate
-
 r
L-aspartate 4-semialdehyde + L-glutamate
-
 Halomonas elongata L-2,4-diaminobutanoate + 2-oxoglutarate
-
 r
L-aspartate 4-semialdehyde + L-homoserine about 5% of the activity with L-glutamate Halomonas elongata L-2,4-diaminobutanoate + 4-hydroxy-2-oxobutanoic acid
-
 r
L-ornithine + 2-oxoglutarate about 6% of the activity with L-2,4-diaminobutanoate Halomonas elongata ? + L-glutamate
-
 r
additional information no substrates: L-alanine, L-aspartate, L-lysine Halomonas elongata ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 44000, SDS-PAGE Halomonas elongata

Synonyms

Synonyms Comment Organism
2,4-diaminobutyrate aminotransferase
-
 Halomonas elongata
DABA aminotransferase
-
 Halomonas elongata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Halomonas elongata

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Halomonas elongata

pI Value

Organism Comment pI Value Maximum pI Value
Halomonas elongata isoelectric focussing
-
 6.2